ArticleWorld journal of gastroenterology2011
Proteome of human colon cancer stem cells: a comparative analysis.
Article in World journal of gastroenterology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Cell Line-Dependent Cell Death Pathways Induced by Thymoquinone in Colorectal Cancer Cells.Molecules (Basel, Switzerland) · 2026Article
- Tissue-specific cancer stem/progenitor cells: Therapeutic implications.World journal of stem cells · 2023Review
- CEACAM5 overexpression is a reliable characteristic of CD133-positive colorectal cancer stem cells.Cancer biomarkers : section A of Disease markers · 2021Article
- Novel Prognostic Biomarkers in Metastatic and Locally Advanced Colorectal Cancer: Micronuclei Frequency and Telomerase Activity in Peripheral Blood Lymphocytes.Frontiers in oncology · 2021Article
- HPVTranslational oncology · 2021Review
- Cancer stem cells in progression of colorectal cancer.Oncotarget · 2018Review
- Major Protein of Carcinoembryonic Antigen Gene Family - CD66c, A Novel Marker in Colon Carcinoma.Journal of clinical and diagnostic research : JCDR · 2016Article
- Proteomic analysis of CD44(+) and CD44(-) gastric cancer cells.Molecular and cellular biochemistry · 2014Article
- Review of application of mass spectrometry for analyses of anterior eye proteome.World journal of biological chemistry · 2014Review
- Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells.World journal of gastroenterology · 2013Article
- Colorectal cancer biomarkers and the potential role of cancer stem cells.Journal of Cancer · 2013Article
- Aberrant expression of CD133 in non-small cell lung cancer and its relationship to vasculogenic mimicry.BMC cancer · 2012Article
- Phosphoproteome of human glioblastoma initiating cells reveals novel signaling regulators encoded by the transcriptome.PloS one · 2012Article
- A Pilot Study Assessing the Potential Role of non-CD133 Colorectal Cancer Stem Cells as Biomarkers.Journal of Cancer · 2012Article
- miR-93 suppresses proliferation and colony formation of human colon cancer stem cells.World journal of gastroenterology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.
methodsSW1116 cells were isolated and cultured with a serum-free medium (SFM). Sphere formation was assayed to observe the formation of colon cancer stem cell spheres. SW1116 cells were inoculated into a serum-containing medium for observing their differentiation characteristics. Proliferation curve and cross-resistance of SW1116 cells to different drugs were detected by MTT. Percentage of SP cells in SW1116 cells was detected with Hoechst33342 staining. Telomerase activity in SW1116cells was checked by polymerase chain reaction (PCR)-enzyme linked immunosorbent assay. Expressions of stem cell relevant genes and proteins were detected by reverse transcription-PCR and Western blot, respectively. Total protein was isolated from SW1116 cells by two-dimensional gel electrophoresis (2-DE) and differentially expressed proteins were identified by tandem mass spectrometry (MALDI-TOF/TOF).
resultsThe isolated SW1116 cells presented as spheroid and suspension growths in SFM with a strong self-renewal, proliferation, differentiation and drug-resistance ability. The percentage of SP cells in SW1116 cells was 38.9%. The SW1116 cells co-expressed the CD133 and CD29 proteins. The telomerase activity in SW1116 cells was increased. The expressions of different stem cell relevant genes and proteins were detected. The proteomic analysis showed that the 26 protein spots were differently expressed in SW1116 cells and 10 protein spots were identified as ubiquitin fusion-degradation 1-like protein, nuclear chloride channel protein, tubulin β, Raichu404X, stratifin, F-actin capping protein α-1 subunit, eukaryotic translation elongation factor 1 delta isoform 2, hypothetical protein, glyceraldehyde-3-phosphate dehydrogenase and guanine nucleotide binding protein β polypeptide 2-like 1, respectively.
conclusionSW1116 cells are biologically characterized by self-renewal, proliferation and differentiation, and the differently expressed proteins in SW1116 cells may be essential for isolating cancer stem cells.
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