ArticleAmerican journal of respiratory cell and molecular biology2011
Hypoxia-inducible factor regulates expression of surfactant protein in alveolar type II cells in vitro.
Article in American journal of respiratory cell and molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- sPLAScience advances · 2026Article
- The Role and Mechanism of HIF-1α in Regulating A549 Alveolar Epithelial Cell Function Under Hypoxic Conditions.Canadian respiratory journal · 2026Article
- Endogenous gene editing of alveolar organoids reveals that expression of pathogenic variant SFTPC-I73T disrupts endosomal function, epithelial polarity and wound healing.bioRxiv : the preprint server for biology · 2025Article
- Article
- Dexamethasone restores TNFα-induced epithelial barrier dysfunction in primary rat alveolar epithelial cells.PloS one · 2023Article
- Cross-Species Insights Into Genomic Adaptations to Hypoxia.Frontiers in genetics · 2020Review
- Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells.PloS one · 2020Article
- Transitional human alveolar type II epithelial cells suppress extracellular matrix and growth factor gene expression in lung fibroblasts.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Pulmonary surfactant-associated proteins and inflammatory factors in obstructive sleep apnea.Sleep & breathing = Schlaf & Atmung · 2018Article
- Potential inflammatory markers in obstructive sleep apnea-hypopnea syndrome.Bosnian journal of basic medical sciences · 2017Article
- Structural and molecular regulation of lung maturation by intratracheal vascular endothelial growth factor administration in the normally grown and placentally restricted fetus.The Journal of physiology · 2016Article
- Stanniocalcin-1 is induced by hypoxia inducible factor in rat alveolar epithelial cells.Biochemical and biophysical research communications · 2014Article
- Innate immune response of human alveolar type II cells infected with severe acute respiratory syndrome-coronavirus.American journal of respiratory cell and molecular biology · 2013Article
- Roflumilast-N-oxide induces surfactant protein expression in human alveolar epithelial cells type II.PloS one · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Alveolar type II (ATII) cells cultured at an air-liquid (A/L) interface maintain differentiation, but they lose these properties when they are submerged. Others showed that an oxygen tension gradient develops in the culture medium as ATII cells consume oxygen. Therefore, we wondered whether hypoxia inducible factor (HIF) signaling could explain differences in the phenotypes of ATII cells cultured under A/L interface or submerged conditions. ATII cells were isolated from male Sprague-Dawley rats and cultured on inserts coated with a mixture of rat-tail collagen and Matrigel, in medium including 5% rat serum and 10 ng/ml keratinocyte growth factor, with their apical surfaces either exposed to air or submerged. The A/L interface condition maintained the expression of surfactant proteins, whereas that expression was down-regulated under the submerged condition, and the effect was rapid and reversible. Under submerged conditions, there was an increase in HIF1α and HIF2α in nuclear extracts, mRNA levels of HIF inducible genes, vascular endothelial growth factor, glucose transporter-1 (GLUT1), and the protein level of pyruvate dehydrogenase kinase isozyme-1. The expression of surfactant proteins was suppressed and GLUT1 mRNA levels were induced when cells were cultured with 1 mM dimethyloxalyl glycine. The expression of surfactant proteins was restored under submerged conditions with supplemented 60% oxygen. HIF signaling and oxygen tension at the surface of cells appears to be important in regulating the phenotype of rat ATII cells.
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