ArticleThe Journal of biological chemistry2011
Protein interactions of phosphatase and tensin homologue (PTEN) and its cancer-associated G20E mutant compared by using stable isotope labeling by amino acids in cell culture-based parallel affinity purification.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Transcriptome-(phospho)proteome characterization of brain of a germline model of cytoplasmic-predominant Pten expression with autism-like phenotypes.NPJ genomic medicine · 2021Article
- Regulation and modulation of PTEN activity.Molecular biology reports · 2018Review
- Article
- The important role of the receptor for activated C kinase 1 (RACK1) in nasopharyngeal carcinoma progression.Journal of translational medicine · 2016Article
- Proteomics-Based Analysis of Protein Complexes in Pluripotent Stem Cells and Cancer Biology.International journal of molecular sciences · 2016Review
- IQGAP1 is a phosphoinositide effector and kinase scaffold.Advances in biological regulation · 2016Review
- The biology of IQGAP proteins: beyond the cytoskeleton.EMBO reports · 2015Review
- IQGAPs choreograph cellular signaling from the membrane to the nucleus.Trends in cell biology · 2015Review
- PTEN function: the long and the short of it.Trends in biochemical sciences · 2014Review
- A mass spectrometry view of stable and transient protein interactions.Advances in experimental medicine and biology · 2014Review
- Rescue of glandular dysmorphogenesis in PTEN-deficient colorectal cancer epithelium by PPARγ-targeted therapy.Oncogene · 2013Article
- Proteomics-based methods for discovery, quantification, and validation of protein-protein interactions.Analytical chemistry · 2013Review
- Mutant p53 interactome identifies nardilysin as a p53R273H-specific binding partner that promotes invasion.EMBO reports · 2012Article
- The functions and regulation of the PTEN tumour suppressor.Nature reviews. Molecular cell biology · 2012Review
- IQGAP1 and its binding proteins control diverse biological functions.Cellular signalling · 2012Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tumor suppressor PTEN (phosphatase and tensin homologue) negatively regulates the PI3K pathway through its lipid phosphatase activity and is one of the most commonly lost tumor suppressors in human cancers. Though the tumor suppressive function involves the lipid phosphatase-dependent and -independent activities of PTEN, the mechanism leading to the phosphatase-independent function of PTEN is understood poorly. Some PTEN mutants have lipid phosphatase activity but fail to suppress cell growth. Here, we use a cancer-associated mutant, G20E, to gain insight into the phosphatase-independent function of PTEN by investigating protein-protein interactions using MS-based stable isotope labeling by amino acids in cell culture (SILAC). A strategy named parallel affinity purification (PAP) and SILAC has been developed to prioritize interactors and to compare the interactions between wild-type and G20E PTEN. Clustering of the prioritized interactors acquired by the PAP-SILAC approach shows three distinct clusters: 1) wild-type-specific interactors, 2) interactors unique to the G20E mutant, and 3) proteins common to wild-type and mutant. These interactors are involved mainly in cell migration and apoptosis pathways. We further demonstrate that the wild-type-specific interactor, NUDTL16L1, is required for the regulatory function of wild-type PTEN in cell migration. These findings contribute to a better understanding of the mechanisms of the phosphatase-dependent and -independent functions of PTEN.
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