ArticleThe Journal of biological chemistry2011
Structure-function analysis of the C-terminal domain of CNM67, a core component of the Saccharomyces cerevisiae spindle pole body.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- MHC Class I Ligands of Rhesus Macaque Killer Cell Ig-like Receptors.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.eLife · 2021Article
- Anatomy of the fungal microtubule organizing center, the spindle pole body.Current opinion in structural biology · 2021Review
- Structure and function of Spc42 coiled-coils in yeast centrosome assembly and duplication.Molecular biology of the cell · 2019Article
- Bipolar switching by HCN voltage sensor underlies hyperpolarization activation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Integrative structure modeling with the Integrative Modeling Platform.Protein science : a publication of the Protein Society · 2018Article
- Design considerations in coiled-coil fusion constructs for the structural determination of a problematic region of the human cardiac myosin rod.Journal of structural biology · 2017Article
- The molecular architecture of the yeast spindle pole body core determined by Bayesian integrative modeling.Molecular biology of the cell · 2017Article
- Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment.The Journal of cell biology · 2017Article
- Structure and dynamics underlying elementary ligand binding events in human pacemaking channels.eLife · 2016Article
- Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.The Journal of biological chemistry · 2016Article
- Article
- Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Kinesin-2 KIF3AB exhibits novel ATPase characteristics.The Journal of biological chemistry · 2014Article
- Structural organization of FtsB, a transmembrane protein of the bacterial divisome.Biochemistry · 2013Article
- Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.The Journal of cell biology · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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Abstract
The spindle pole body of the budding yeast Saccharomyces cerevisiae has served as a model system for understanding microtubule organizing centers, yet very little is known about the molecular structure of its components. We report here the structure of the C-terminal domain of the core component Cnm67 at 2.3 Å resolution. The structure determination was aided by a novel approach to crystallization of proteins containing coiled-coils that utilizes globular domains to stabilize the coiled-coils. This enhances their solubility in Escherichia coli and improves their crystallization. The Cnm67 C-terminal domain (residues Asn-429-Lys-581) exhibits a previously unseen dimeric, interdigitated, all α-helical fold. In vivo studies demonstrate that this domain alone is able to localize to the spindle pole body. In addition, the structure reveals a large functionally indispensable positively charged surface patch that is implicated in spindle pole body localization. Finally, the C-terminal eight residues are disordered but are critical for protein folding and structural stability.
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