ArticleThe Journal of biological chemistry2011
Lipids and topological rules of membrane protein assembly: balance between long and short range lipid-protein interactions.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Exploring Uniform, Dual, and Dynamic Topologies of Membrane Proteins by Substituted Cysteine Accessibility Method (SCAM™).Methods in molecular biology (Clifton, N.J.) · 2024Article
- How physical forces drive the process of helical membrane protein folding.EMBO reports · 2022Review
- Eugene P. Kennedy's Legacy: Defining Bacterial Phospholipid Pathways and Function.Frontiers in molecular biosciences · 2021Review
- How bilayer properties influence membrane protein folding.Protein science : a publication of the Protein Society · 2020Review
- Structural and Functional Adaptability of Sucrose and Lactose Permeases fromBiochemistry · 2020Article
- The lipid-dependent structure and function of LacY can be recapitulated and analyzed in phospholipid-containing detergent micelles.Scientific reports · 2019Article
- Lipid-Assisted Membrane Protein Folding and Topogenesis.The protein journal · 2019Review
- Effects of mixed proximal and distal topogenic signals on the topological sensitivity of a membrane protein to the lipid environment.Biochimica et biophysica acta. Biomembranes · 2017Article
- Complete topology inversion can be part of normal membrane protein biogenesis.Protein science : a publication of the Protein Society · 2017Article
- Dynamic Lipid-dependent Modulation of Protein Topology by Post-translational Phosphorylation.The Journal of biological chemistry · 2017Article
- In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers.Scientific reports · 2016Article
- Model parameters for simulation of physiological lipids.Journal of computational chemistry · 2016Article
- Substrate Selectivity of Lysophospholipid Transporter LplT Involved in Membrane Phospholipid Remodeling in Escherichia coli.The Journal of biological chemistry · 2016Article
- Mutual Interactions between Aquaporins and Membrane Components.Frontiers in plant science · 2016Review
- Dynamic membrane protein topological switching upon changes in phospholipid environment.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Lipids and topological rules governing membrane protein assembly.Biochimica et biophysica acta · 2014Review
- In vitro reconstitution of lipid-dependent dual topology and postassembly topological switching of a membrane protein.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A retrospective: use of Escherichia coli as a vehicle to study phospholipid synthesis and function.Biochimica et biophysica acta · 2013Review
- Proper fatty acid composition rather than an ionizable lipid amine is required for full transport function of lactose permease from Escherichia coli.The Journal of biological chemistry · 2013Article
- Prediction of lipid-binding regions in cytoplasmic and extracellular loops of membrane proteins as exemplified by protein translocation membrane proteins.The Journal of membrane biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The N-terminal six-transmembrane domain (TM) bundle of lactose permease of Escherichia coli is uniformly inverted when assembled in membranes lacking phosphatidylethanolamine (PE). Inversion is dependent on the net charge of cytoplasmically exposed protein domains containing positive and negative residues, net charge of the membrane surface, and low hydrophobicity of TM VII acting as a molecular hinge between the two halves of lactose permease (Bogdanov, M., Xie, J., Heacock, P., and Dowhan, W. (2008) J. Cell Biol. 182, 925-935). Net neutral lipids suppress the membrane translocation potential of negatively charged amino acids, thus increasing the cytoplasmic retention potential of positively charged amino acids. Herein, TM organization of sucrose permease (CscB) and phenylalanine permease (PheP) as a function of membrane lipid composition was investigated to extend these principles to other proteins. For CscB, topological dependence on PE only becomes evident after a significant increase in the net negative charge of the cytoplasmic surface of the N-terminal TM bundle. High negative charge is required to overcome the thermodynamic block to inversion due to the high hydrophobicity of TM VII. Increasing the positive charge of the cytoplasmic surface of the N-terminal TM hairpin of PheP, which is misoriented in PE-lacking cells, favors native orientation in the absence of PE. PheP and CscB also display co-existing dual topologies dependent on changes in the charge balance between protein domains and the membrane lipids. Therefore, the topology of both permeases is dependent on PE. However, CscB topology is governed by thermodynamic balance between opposing lipid-dependent electrostatic and hydrophobic interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.