ArticleThe Journal of biological chemistry2011
Recognition of gram-positive intestinal bacteria by hybridoma- and colostrum-derived secretory immunoglobulin A is mediated by carbohydrates.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 107 citations in OpenAlex.
- Disruption of IgA-mediated aggregation at weaning favors mucus encroachment by commensal bacteria.NPJ biofilms and microbiomes · 2026Article
- Gut microbial IgA coating in infants with traditional farming lifestyle and urban infants with allergic outcomes.Frontiers in immunology · 2026Article
- Ig-microbiota binding patterns in mothers and infants: a scoping review.Gut microbiome (Cambridge, England) · 2026Review
- Coexist or eliminate? Antibody-based functional silencing versus eradication in gut microbiome- targeted therapy.Frontiers in immunology · 2026Review
- Clinical features and risk factors of abdominal IgA vasculitis in adults.Clinical rheumatology · 2025Article
- Bacterial and host fucosylation maintain IgA homeostasis to limit intestinal inflammation in mice.Nature microbiology · 2025Article
- Immunoglobulin secretion influences the composition of chicken caecal microbiota.Scientific reports · 2024Article
- Article
- Exploring the memory of the gut microbiome: a multifaceted perspective.Frontiers in microbiomes · 2024Article
- Development of Orally Ingestible IgA Antibody Drugs to Maintain Symbiosis Between Humans and Microorganisms.Advances in experimental medicine and biology · 2024Article
- The role of the glycome in symbiotic host-microbe interactions.Glycobiology · 2023Review
- Gut microbiome and breast-feeding: Implications for early immune development.The Journal of allergy and clinical immunology · 2022Review
- Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother-Infant Pairs.International journal of molecular sciences · 2022Article
- Review
- The Study of Microbe-Host Two-Way Communication.Microorganisms · 2022Article
- Advances in IgA glycosylation and its correlation with diseases.Frontiers in chemistry · 2022Review
- Optimization of a human milk-directed quantitative sIgA ELISA method substantiated by mass spectrometry.Analytical and bioanalytical chemistry · 2021Article
- Diversity and dynamism of IgA-microbiota interactions.Nature reviews. Immunology · 2021Review
- Microbiota-antibody interactions that regulate gut homeostasis.Cell host & microbe · 2021Review
- Active free secretory component and secretory IgA in human milk: do maternal vaccination, allergy, infection, mode of delivery, nutrition and active lifestyle change their concentrations?Pediatric research · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Humans live in symbiosis with 10(14) commensal bacteria among which >99% resides in their gastrointestinal tract. The molecular bases pertaining to the interaction between mucosal secretory IgA (SIgA) and bacteria residing in the intestine are not known. Previous studies have demonstrated that commensals are naturally coated by SIgA in the gut lumen. Thus, understanding how natural SIgA interacts with commensal bacteria can provide new clues on its multiple functions at mucosal surfaces. Using fluorescently labeled, nonspecific SIgA or secretory component (SC), we visualized by confocal microscopy the interaction with various commensal bacteria, including Lactobacillus, Bifidobacteria, Escherichia coli, and Bacteroides strains. These experiments revealed that the interaction between SIgA and commensal bacteria involves Fab- and Fc-independent structural motifs, featuring SC as a crucial partner. Removal of glycans present on free SC or bound in SIgA resulted in a drastic drop in the interaction with gram-positive bacteria, indicating the essential role of carbohydrates in the process. In contrast, poor binding of gram-positive bacteria by control IgG was observed. The interaction with gram-negative bacteria was preserved whatever the molecular form of protein partner used, suggesting the involvement of different binding motifs. Purified SIgA and SC from either mouse hybridoma cells or human colostrum exhibited identical patterns of recognition for gram-positive bacteria, emphasizing conserved plasticity between species. Thus, sugar-mediated binding of commensals by SIgA highlights the currently underappreciated role of glycans in mediating the interaction between a highly diverse microbiota and the mucosal immune system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.