ArticleThe Journal of allergy and clinical immunology2011
Human mast cell degranulation and preformed TNF secretion require mitochondrial translocation to exocytosis sites: relevance to atopic dermatitis.
Article in The Journal of allergy and clinical immunology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 137 citations in OpenAlex.
- Therapeutic potential of phloridzin carbomer gel for skin inflammatory healing in atopic dermatitis.Archives of dermatological research · 2025Article
- Oxidative Stress and Mitochondria Are Involved in Anaphylaxis and Mast Cell Degranulation: A Systematic Review.Antioxidants (Basel, Switzerland) · 2024Review
- Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion.Journal of cellular and molecular medicine · 2024Article
- Impaired Proliferation of CD8Journal of immunology research · 2024Article
- Mitochondria: the gatekeepers between metabolism and immunity.Frontiers in immunology · 2024Review
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- Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis.Immunity, inflammation and disease · 2023Article
- Potential Role of Moesin in Regulating Mast Cell Secretion.International journal of molecular sciences · 2023Review
- Article
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- The Distinct Effects of the Mitochondria-Targeted STAT3 Inhibitors Mitocur-1 and Mitocur-3 on Mast Cell and Mitochondrial Functions.International journal of molecular sciences · 2023Article
- Role of UPRFrontiers in cellular and infection microbiology · 2023Review
- Dynamin-related protein 1 differentially regulates FcεRI- and substance P-induced mast cell activation.The Journal of allergy and clinical immunology · 2022Article
- Mast Cell Cytokines in Acute and Chronic Gingival Tissue Inflammation: Role of IL-33 and IL-37.International journal of molecular sciences · 2022Review
- Advances in the modulation of ROS and transdermal administration for anti-psoriatic nanotherapies.Journal of nanobiotechnology · 2022Review
- Modulating donor mitochondrial fusion/fission delivers immunoprotective effects in cardiac transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022Article
- Chronic allergy signaling: is it all stressed-out mitochondria?Faculty reviews · 2022Review
- Skin Commensal Bacteria Modulates the Immune Balance of Mice to Alleviate Atopic Dermatitis-Induced Damage.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Psoriasiform Inflammation Is Associated with Mitochondrial Fission/GDAP1L1 Signaling in Macrophages.International journal of molecular sciences · 2021Article
- Alterations in mitochondrial morphology as a key driver of immunity and host defence.EMBO reports · 2021Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundMast cells derive from hematopoietic cell precursors and participate in tissue allergic, immune, and inflammatory processes. They secrete many mediators, including preformed TNF, in response to allergic, neuropeptide, and environmental triggers. However, regulation of mast cell degranulation is not well understood.
objectiveWe investigated the role of mitochondrial dynamics in degranulation of human cultured mast cells.
methodsHuman umbilical cord blood-derived mast cells (hCBMCs) and Laboratory of Allergic Diseases 2 (LAD2) mast cells were examined by confocal and differential interference contrast microscopy during activation by IgE/antigen and substance P (SP). Mast cells in control and atopic dermatitis (AD) skin were evaluated by transmission electron microscopy. LAD2 cells were pretreated with mitochondrial division inhibitor, a dynamin-related protein 1 (Drp1) inhibitor, and small interfering RNA for Drp1, which is necessary for mitochondrial fission and translocation. Calcineurin and Drp1 gene expression was analyzed in stimulated LAD2 cells and AD skin biopsies.
resultsStimulation of hCBMCs with IgE/antigen or LAD2 cells with SP leads to rapid (30 minutes) secretion of preformed TNF. Degranulation is accompanied by mitochondrial translocation from a perinuclear location to exocytosis sites. Extracellular calcium depletion prevents these effects, indicating calcium requirement. The calcium-dependent calcineurin and Drp1 are activated 30 minutes after SP stimulation. Reduction of Drp1 activity by mitochondrial division inhibitor and decrease of Drp1 expression using small interfering RNA inhibit mitochondrial translocation, degranulation, and TNF secretion. Mitochondrial translocation is also evident by transmission electron microscopy in skin mast cells from AD biopsies, in which gene expression of calcineurin, Drp1, and SP is higher than in normal skin.
conclusionHuman mast cell degranulation requires mitochondrial dynamics, also implicated in AD.
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