ArticleActa veterinaria Scandinavica2011
Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera.
Article in Acta veterinaria Scandinavica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Regular outdoor access decreases stress with positive effects on the health of growing pigs raised on a conventional farm.Scientific reports · 2026Article
- Lawsonia intracellularis regulates nuclear factor-κB signalling pathway during infection.PloS one · 2024Article
- Development and validation of a flow cytometry antibody test forFrontiers in immunology · 2023Article
- Editorial: Flow cytometry - A powerful tool for diagnosis and therapy monitoring in hematology and immunology.Frontiers in medicine · 2023Article
- Serological Assessment ofPathogens (Basel, Switzerland) · 2022Article
- Review of methods for the detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Review
- Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick.BMC veterinary research · 2019Article
- Factors associated with the growing-finishing performances of swine herds: an exploratory study on serological and herd level indicators.Porcine health management · 2018Article
- Seroprevalence of Lawsonia intracellularis antibodies in intensive pig farms in China.BMC veterinary research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLawsonia intracellularis is a common cause of chronic diarrhoea and poor performance in young growing pigs. Diagnosis of this obligate intracellular bacterium is based on the demonstration of the microbe or microbial DNA in tissue specimens or faecal samples, or the demonstration of L. intracellularis-specific antibodies in sera. The aim of the present study was to evaluate a blocking ELISA in the detection of serum antibodies to L. intracellularis, by comparison to the previously widely used immunofluorescent antibody test (IFAT).
methodsSera were collected from 176 pigs aged 8-12 weeks originating from 24 herds with or without problems with diarrhoea and poor performance in young growing pigs. Sera were analyzed by the blocking ELISA and by IFAT. Bayesian modelling techniques were used to account for the absence of a gold standard test and the results of the blocking ELISA was modelled against the IFAT test with a "2 dependent tests, 2 populations, no gold standard" model.
resultsAt the finally selected cut-off value of percent inhibition (PI) 35, the diagnostic sensitivity of the blocking ELISA was 72% and the diagnostic specificity was 93%. The positive predictive value was 0.82 and the negative predictive value was 0.89, at the observed prevalence of 33.5%.
conclusionThe sensitivity and specificity as evaluated by Bayesian statistic techniques differed from that previously reported. Properties of diagnostic tests may well vary between countries, laboratories and among populations of animals. In the absence of a true gold standard, the importance of validating new methods by appropriate statistical methods and with respect to the target population must be emphasized.
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