ArticleGene therapy2011
Explorations of high-intensity therapeutic ultrasound and microbubble-mediated gene delivery in mouse liver.
Article in Gene therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Effective gene immunotherapy for melanoma utilizing an advancedMolecular therapy. Oncology · 2025Article
- The current progress and futural prospects of ultrasound-targeted microbubble destruction for liver diseases: a narrative review.Translational gastroenterology and hepatology · 2025Review
- Ultrasound-mediated gene delivery specifically targets liver sinusoidal endothelial cells for sustained FVIII expression in hemophilia A mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Delivery of gene editing therapeutics.Nanomedicine : nanotechnology, biology, and medicine · 2023Review
- Ultrasound-mediated gene delivery of factor VIII plasmids for hemophilia A gene therapy in mice.Molecular therapy. Nucleic acids · 2022Article
- A Comparison of Focused and Unfocused Ultrasound for Microbubble-Mediated Gene Delivery.Ultrasound in medicine & biology · 2021Article
- Transcutaneous Ultrasound-Mediated Nonviral Gene Delivery to the Liver in a Porcine Model.Molecular therapy. Methods & clinical development · 2019Article
- Applications of Ultrasound to Stimulate Therapeutic Revascularization.International journal of molecular sciences · 2019Review
- Application of Direct Sonoporation from a Defined Surface Area of the Peritoneum: Evaluation of Transfection Characteristics in Mice.Pharmaceutics · 2019Article
- Ultrasound-Mediated Gene Therapy in Swine Livers Using Single-Element, Multi-lensed, High-Intensity Ultrasound Transducers.Molecular therapy. Methods & clinical development · 2018Article
- Prolonging pulse duration in ultrasound-mediated gene delivery lowers acoustic pressure threshold for efficient gene transfer to cells and small animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- [Enhancement of gene transfection efficiency and therapeutic effect of ultrasound-targeted microbubble destructionZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Article
- Circulating Magnetic Microbubbles for Localized Real-Time Control of Drug Delivery by Ultrasonography-Guided Magnetic Targeting and Ultrasound.Theranostics · 2018Article
- Ultrasound-targeted microbubble destruction in gene therapy: A new tool to cure human diseases.Genes & diseases · 2017Review
- [Research of enhanced green fluorescent protein gene transfer with ultrasound-mediated microbubble destruction in bone defects].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017Article
- Thermal Assisted In Vivo Gene Electrotransfer.Current gene therapy · 2016Article
- Article
- Evaluation of in vivo antitumor effects of ANT2 shRNA delivered using PEI and ultrasound with microbubbles.Gene therapy · 2015Article
- Development of therapeutic microbubbles for enhancing ultrasound-mediated gene delivery.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Lipid nanoparticles for gene delivery.Advances in genetics · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Ultrasound (US) combined with microbubbles (MBs) is a promising technology for non-viral gene delivery. Significant enhancements of gene expression have been obtained in our previous studies. To optimize and prepare for application to larger animal models, the luciferase reporter gene transfer efficacy of lipid-based Definity MBs of various concentrations, pressure amplitudes and a novel unfocused high-intensity therapeutic US (HITU) system were explored. Luciferase expression exhibited a dependence on MB dose over the range of 0-25 vol%, and a strong dependence on acoustic peak negative pressure at over the range of 0-3.2 MPa. Gene expression reached an apparent plateau at MB concentration ≥2.5 vol% or at negative pressures >1.8 MPa. Maximum gene expression in treated animals was 700-fold greater than in negative controls. Pulse train US exposure protocols produced an upward trend of gene expression with increasing quiescent time. The hyperbolic correlation of gene expression and transaminase levels suggested that an optimum gene delivery effect can be achieved by maximizing acoustic cavitation-induced enhancement of DNA uptake and minimizing unproductive tissue damage. This study validated the new HITU system equipped with an unfocused transducer with a larger footprint capable of scanning large tissue areas to effectively enhance gene transfer efficiencies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.