ArticleJournal of biosciences2011
Heat-inducible Cre-lox system for marker excision in transgenic rice.
Article in Journal of biosciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- CRISPR/Cas9-driven double modification of grapevine MLO6-7 imparts powdery mildew resistance, while editing of NPR3 augments powdery and downy mildew tolerance.The Plant journal : for cell and molecular biology · 2025Article
- Comparing four heat-inducible promoters in stably transformed sugarcane regarding spatial and temporal control of transgene expression reveals candidates to drive stem-preferred transgene expression.Frontiers in plant science · 2025Article
- Selectable Markers to Marker-Free Selection in Rice.Molecular biotechnology · 2022Review
- An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice.International journal of molecular sciences · 2022Article
- A Prospective Review on Selectable Marker-Free Genome Engineered Rice: Past, Present and Future Scientific Realm.Frontiers in genetics · 2022Review
- Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells.Nature communications · 2021Article
- The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa).BMC plant biology · 2021Article
- Article
- Sequential Genome Editing and Induced Excision of the Transgene inFrontiers in plant science · 2020Article
- Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector.Genes · 2019Article
- Article
- Heat-Shock-Induced Removal of Transgenes Using the Gene-Deletor System in Hybrid Aspen (Genes · 2018Article
- A new location to split Cre recombinase for protein fragment complementation.Plant biotechnology journal · 2017Article
- A Chemical-Induced, Seed-Soaking Activation Procedure for Regulated Gene Expression in Rice.Frontiers in plant science · 2017Article
- Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi.Transgenic research · 2016Article
- Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).Plant cell reports · 2016Article
- Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion.BMC biotechnology · 2015Article
- Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum.Molecules (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study assessed the efficacy of a heat-inducible cre gene for conditional removal of the marker gene from a rice genome via Cre-lox recombination. A cre gene controlled by the soybean heat-shock promoter was introduced into the rice genome along with the recombination target (lox) construct. Cre-mediated recombination was expected to remove the marker gene and activate the promoter-less GUS gene. Six transgenic lines displayed well-regulated heat-inducible Cre activity in the callus. However, only one line that contained a single copy of the cre gene maintained this property in the regenerated plants and their progeny. Marker-free progeny were obtained from the plant that was heat-treated at the seedling stage, indicating the inheritance of the recombination 'footprint'. The presence of the 'footprint' was verified by polymerase chain reaction and Southern analysis. Therefore, the cre gene controlled by the soybean heat-shock promoter is an effective tool for conditional removal of the marker gene in rice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.