ArticleAmerican journal of physiology. Cell physiology2011
Improving murine embryonic stem cell differentiation into cardiomyocytes with neuregulin-1: differential expression of microRNA.
Article in American journal of physiology. Cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Functional impairment of human resident cardiac stem cells by the cardiotoxic antineoplastic agent trastuzumab.Stem cells translational medicine · 2012Trial
- NRG1β-overexpressing mesenchymal stem cell-derived exosomes alleviate oxygen-glucose deprivation-mediated neuronal injury via the miR-296-3p/MAOA axis.Frontiers in cellular neuroscience · 2026Article
- Prophylactic Evidence of MSCs-Derived Exosomes in Doxorubicin/Trastuzumab-Induced Cardiotoxicity: Beyond Mechanistic Target of NRG-1/Erb Signaling Pathway.International journal of molecular sciences · 2022Article
- Post-Transcriptional Regulation of Molecular Determinants during Cardiogenesis.International journal of molecular sciences · 2022Review
- Analysis of miRNAs Involved in Mouse Heart Injury Upon Coxsackievirus A2 Infection.Frontiers in cellular and infection microbiology · 2022Article
- MiRNA Signaling in Viral Myocarditis Novel and Unique Pathological Features.Acta Cardiologica Sinica · 2018Article
- High glucose suppresses embryonic stem cell differentiation into cardiomyocytes : High glucose inhibits ES cell cardiogenesis.Stem cell research & therapy · 2016Article
- Co-existence of intact stemness and priming of neural differentiation programs in mES cells lacking Trim71.Scientific reports · 2015Article
- Neuregulin-1β induces mature ventricular cardiac differentiation from induced pluripotent stem cells contributing to cardiac tissue repair.Stem cells and development · 2015Article
- The roles of neuregulin-1 in cardiac development, homeostasis, and disease.Biomarker insights · 2015Review
- Neuregulin-1β induces embryonic stem cell cardiomyogenesis via ErbB3/ErbB2 receptors.The Biochemical journal · 2014Article
- Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction.Experimental and therapeutic medicine · 2014Article
- Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens.Journal of cellular and molecular medicine · 2013Article
- The role of neuregulin/ErbB2/ErbB4 signaling in the heart with special focus on effects on cardiomyocyte proliferation.American journal of physiology. Heart and circulatory physiology · 2012Review
- MicroRNAs 296 and 298 are imprinted and part of the GNAS/Gnas cluster and miR-296 targets IKBKE and Tmed9.RNA (New York, N.Y.) · 2012Article
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5 authors at 3 institutions in 1 country.
Funding
Abstract
Identification of factors that direct embryonic stem (ES) cell (ESC) differentiation into functional cardiomyocytes is essential for successful use of ESC-based therapy for cardiac repair. Neuregulin-1 (NRG1) and microRNA play important roles in the cardiac differentiation of ESCs. Understanding how NRG1 regulates microRNA will provide new mechanistic insights into the role of NRG1 on ESCs. It may also lead to the discovery of novel microRNAs that are important for ESC cardiac differentiation. The objective of this study was to assess the microRNA expression profile during NRG1-induced ESC cardiac differentiation. Murine ESCs were incubated with a recombinant NRG1β or an inhibitor of ErbB2 or ErbB4 during hanging drop-induced cardiac differentiation. The expression of cardiac-specific markers and microRNAs was analyzed by RT-PCR and microRNA array, respectively. We found that the expression of NRG1 and the ErbB receptors was increased during hanging drop-induced cardiac differentiation of ESCs. NRG1 stimulation during a specific developmental window enhanced, while inhibition of the ErbB2 or ErbB4 receptor inhibited, cardiac differentiation of ESCs. NRG1 increased the expression of mmu-miR-296-3p and mmu-miR-200c*, and decreased mmu-miR-465b-5p. Inhibition of mmu-miR-296-3p or mmu-miR-200c* decreased, while inhibition of mmu-miR-465-5p increased, the differentiation of ESCs into the cardiac lineage. This is the first report demonstrating that microRNAs are differentially regulated by NRG1-ErbB signaling during cardiac differentiation of ESCs. This study has also identified new microRNAs that are important for ESC cardiac differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.