ArticleBiochemistry2011
Effects of HIV-1 Nef on human N-myristoyltransferase 1.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Vir2Drug: a drug repurposing framework based on protein similarities between pathogens.Briefings in bioinformatics · 2023Article
- Defective HIV-1 proviruses produce viral proteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding.Journal of molecular biology · 2018Article
- Creation of a Long-Acting Nanoformulated 2',3'-Dideoxy-3'-Thiacytidine.Journal of acquired immune deficiency syndromes (1999) · 2017Article
- N-Lauroylation during the Expression of Recombinant N-Myristoylated Proteins: Implications and Solutions.Chembiochem : a European journal of chemical biology · 2016Article
- Membrane-Associated Conformation of HIV-1 Nef Investigated with Hydrogen Exchange Mass Spectrometry at a Langmuir Monolayer.Analytical chemistry · 2015Article
- Hydrogen Exchange Mass Spectrometry of Proteins at Langmuir Monolayers.Analytical chemistry · 2015Article
- Article
- Heterologous Src homology 4 domains support membrane anchoring and biological activity of HIV-1 Nef.The Journal of biological chemistry · 2014Article
- The ABCA1 domain responsible for interaction with HIV-1 Nef is conformational and not linear.Biochemical and biophysical research communications · 2014Article
- Conformational transition of membrane-associated terminally acylated HIV-1 Nef.Structure (London, England : 1993) · 2013Article
- Minimizing carry-over in an online pepsin digestion system used for the H/D exchange mass spectrometric analysis of an IgG1 monoclonal antibody.Journal of the American Society for Mass Spectrometry · 2012Article
- Characterization and solution structure of mouse myristoylated methionine sulfoxide reductase A.The Journal of biological chemistry · 2012Article
- Using hydrogen/deuterium exchange mass spectrometry to study conformational changes in granulocyte colony stimulating factor upon PEGylation.Journal of the American Society for Mass Spectrometry · 2012Article
- Intracellular overexpression of HIV-1 Nef impairs differentiation and maturation of monocytic precursors towards dendritic cells.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The HIV-1 accessory protein Nef is N-terminally myristoylated, and this post-translational modification is essential for Nef function in AIDS progression. Transfer of a myristate group from myristoyl coenzyme A to Nef occurs cotranslationally and is catalyzed by human N-myristoyltransferase 1 (NMT). To investigate the conformational effects of myristoylation on Nef structure as well as to probe the nature of the Nef:NMT complex, we investigated various forms of Nef with hydrogen exchange mass spectrometry. Conformational changes in Nef were not detected as a result of myristoylation, and NMT had no effect on deuterium uptake by Nef in a myrNef:NMT complex. However, myrNef binding did have an effect on NMT deuterium uptake. Major HX differences in NMT were primarily located around the active site, with more subtle differences, at the longer time points, across the structure. At the shortest time point, significant differences between the two states were observed in two regions which interact strongly with the phosphate groups of coenzyme A. On the basis of our results, we propose a model of the Nef:NMT complex in which only the myristoyl moiety holds the two proteins together in complex and speculate that perhaps NMT chaperones Nef to the membrane and thereby protects the myristic acid group from the cytosol rather than Nef operating through a myristoyl switch mechanism.
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Registered trials
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