ArticleThe journal of physical chemistry. B2011
Transitions between elongated conformations of ubiquitin [M+11H]11+ enhance hydrogen/deuterium exchange.
Article in The journal of physical chemistry. B, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Structural differentiation of protein charge state conformers via gas-phase ion/ion cross-linking mass spectrometry.International journal of mass spectrometry · 2025Article
- Accessing Different Protein Conformer Ensembles with Tunable Capillary Vibrating Sharp-Edge Spray Ionization.The journal of physical chemistry. B · 2025Article
- In-droplet hydrogen-deuterium exchange to examine protein/peptide solution conformer heterogeneity.Rapid communications in mass spectrometry : RCM · 2023Article
- Characterizing Thermal Transitions of IgG with Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2019Article
- Comprehensive Gas-Phase Peptide Ion Structure Studies Using Ion Mobility Techniques: Part 2. Gas-Phase Hydrogen/Deuterium Exchange for Ion Population Estimation.Journal of the American Society for Mass Spectrometry · 2017Article
- Folding of Protein Ions in the Gas Phase after Cation-to-Anion Proton-Transfer Reactions.Journal of the American Chemical Society · 2016Article
- Multiple gas-phase conformations of proline-containing peptides: is it always cis/trans isomerization?The Analyst · 2016Article
- Ion Mobility Spectrometry-Hydrogen Deuterium Exchange Mass Spectrometry of Anions: Part 2. Assessing Charge Site Location and Isotope Scrambling.Journal of the American Society for Mass Spectrometry · 2016Article
- Ion mobility-mass spectrometry: time-dispersive instrumentation.Analytical chemistry · 2015Review
- An IMS-IMS threshold method for semi-quantitative determination of activation barriers: Interconversion of prolineInternational journal of mass spectrometry · 2015Article
- Time-resolved pulsed hydrogen/deuterium exchange mass spectrometry probes gaseous proteins structural kinetics.Journal of the American Society for Mass Spectrometry · 2015Article
- Solution dependence of the collisional activation of ubiquitin [M + 7H](7+) ions.Journal of the American Society for Mass Spectrometry · 2014Article
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Authors and funding
3 authors.
Funding
Abstract
Hydrogen/deuterium (H/D) exchange reactions between different elongated conformations of [M + 11H](11+) ions of ubiquitin and D(2)O are studied by a combination of ion mobility spectrometry (IMS) and mass spectrometry techniques. Three conformers (B, C, and D), resolved in the IMS separation, each exchange ∼27 hydrogens upon exposure to 0.06 Torr of D(2)O vapor for ∼35 to 40 ms. However, a region of the IMS spectrum that appears between the C and D states (corresponding to ions that undergo a structural transition during the mobility separation) undergoes substantially more exchanges (∼39 total sites, 44% more than the B, C, and D states). Selection and activation of the individual B, C, and D states reveals that the increased H/D exchange occurs during the transition between structures. Overall, these studies suggest a key process in establishing the maximum exchange levels involves structural transitions, which allow protected sites to be exposed for some fraction of the reaction time. Analysis of changes in exchange levels upon structural transitions can provide insight about common regions of structure that exist in the B, C, and D conformations.
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