ArticleBiochemistry2011
Native electrospray mass spectrometry reveals the nature and stoichiometry of pigments in the FMO photosynthetic antenna protein.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 78 citations in OpenAlex.
- Living on the edge: light-harvesting efficiency and photoprotection in the core of green sulfur bacteria.Physical chemistry chemical physics : PCCP · 2023Article
- Quantum Entanglement and State-Transference in Fenna-Matthews-Olson Complexes: A Post-Experimental Simulation Analysis in the Computational Biology Domain.International journal of molecular sciences · 2023Article
- Biochemical and spectroscopic characterizations of the oligomeric antenna of the coral symbiotic Symbiodiniaceae Fugacium kawagutii.Photosynthesis research · 2022Article
- High-Resolution Native Mass Spectrometry.Chemical reviews · 2022Review
- Redox conditions correlated with vibronic coupling modulate quantum beats in photosynthetic pigment-protein complexes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structural Biology for the Molecular Insight between Aptamers and Target Proteins.International journal of molecular sciences · 2021Review
- Development of a target identification approach using native mass spectrometry.Scientific reports · 2021Article
- Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry.Nature protocols · 2020Article
- Protein-like Enwrapped Perylene Bisimide Chromophore as a Bright Microcrystalline Emitter Material.Angewandte Chemie (International ed. in English) · 2019Article
- The influence of quaternary structure on the stability of Fenna-Matthews-Olson (FMO) antenna complexes.Photosynthesis research · 2019Article
- On uncorrelated inter-monomer Förster energy transfer in Fenna-Matthews-Olson complexes.Journal of the Royal Society, Interface · 2019Article
- Photoprotective, excited-state quenching mechanisms in diverse photosynthetic organisms.The Journal of biological chemistry · 2018Review
- Native Mass Spectrometry, Ion mobility, and Collision-Induced Unfolding Categorize Malaria Antigen/Antibody Binding.Journal of the American Society for Mass Spectrometry · 2017Article
- Structural Characterization of a Thrombin-Aptamer Complex by High Resolution Native Top-Down Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Two-dimensional electronic spectra of the photosynthetic apparatus of green sulfur bacteria.Scientific reports · 2017Article
- Native Mass Spectrometry Characterizes the Photosynthetic Reaction Center Complex from the Purple Bacterium Rhodobacter sphaeroides.Journal of the American Society for Mass Spectrometry · 2017Article
- Evidence for a cysteine-mediated mechanism of excitation energy regulation in a photosynthetic antenna complex.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Native mass spectrometry and ion mobility characterize the orange carotenoid protein functional domains.Biochimica et biophysica acta · 2016Article
- Native FMO-reaction center supercomplex in green sulfur bacteria: an electron microscopy study.Photosynthesis research · 2016Article
- Characterization of native protein complexes using ultraviolet photodissociation mass spectrometry.Journal of the American Chemical Society · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The nature and stoichiometry of pigments in the Fenna-Matthews-Olson (FMO) photosynthetic antenna protein complex were determined by native electrospray mass spectrometry. The FMO antenna complex was the first chlorophyll-containing protein that was crystallized. Previous results indicate that the FMO protein forms a trimer with seven bacteriochlorophyll a in each monomer. This model has long been a working basis to understand the molecular mechanism of energy transfer through pigment/pigment and pigment/protein coupling. Recent results have suggested, however, that an eighth bacteriochlorophyll is present in some subunits. In this report, a direct mass spectrometry measurement of the molecular weight of the intact FMO protein complex clearly indicates the existence of an eighth pigment, which is assigned as a bacteriochlorophyll a by mass analysis of the complex and HPLC analysis of the pigment. The eighth pigment is found to be easily lost during purification, which results in its partial occupancy in the mass spectra of the intact complex prepared by different procedures. The results are consistent with the recent X-ray structural models. The existence of the eighth bacteriochlorophyll a in this model antenna protein gives new insights into the functional role of the FMO protein and motivates the need for new theoretical and spectroscopic assignments of spectral features of the FMO protein.
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