ArticleMolecular & cellular proteomics : MCP2011
In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Fasciclin 2 Cooperates with Discs Large to Maintain Epithelial Architecture.bioRxiv : the preprint server for biology · 2026Article
- The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila.BMC biology · 2023Article
- Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia.Acta neuropathologica communications · 2022Article
- Identification of covalent modifications regulating immune signaling complex composition and phenotype.Molecular systems biology · 2021Article
- Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals.Journal of cell science · 2021Article
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Characterisation of protein isoforms encoded by the Drosophila Glycogen Synthase Kinase 3 gene shaggy.PloS one · 2020Article
- Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration.Nature communications · 2019Article
- The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.Developmental biology · 2018Article
- Co-complex protein membership evaluation using Maximum Entropy on GO ontology and InterPro annotation.Bioinformatics (Oxford, England) · 2018Article
- The canine MHC class Ia allele DLA-88*508:01 presents diverse self- and canine distemper virus-origin peptides of varying length that have a conserved binding motif.Veterinary immunology and immunopathology · 2018Article
- Top-down characterization of endogenous protein complexes with native proteomics.Nature chemical biology · 2018Article
- Engineering the Drosophila Genome for Developmental Biology.Journal of developmental biology · 2017Review
- Time, space, and disorder in the expanding proteome universe.Proteomics · 2017Article
- Transcription factors, chromatin proteins and the diversification of Hemiptera.Insect biochemistry and molecular biology · 2016Article
- Article
- SILAC-iPAC: a quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture.Journal of proteomics · 2015Article
- Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).Molecular & cellular proteomics : MCP · 2015Article
- A re-examination of the selection of the sensory organ precursor of the bristle sensilla of Drosophila melanogaster.PLoS genetics · 2015Article
- Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions.Frontiers in genetics · 2015Review
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11 authors.
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Abstract
Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experiments and isolated these proteins in their native complexes from embryos. We describe a pipeline for determining interactomes by Parallel Affinity Capture (iPAC) and show its use by identifying partners of several protein baits with a range of sizes and subcellular locations. This purification protocol employs the different tags in parallel and involves detailed comparison of resulting mass spectrometry data sets, ensuring the interaction lists achieved are of high confidence. We show that this approach identifies known interactors of bait proteins as well as novel interaction partners by comparing data achieved with published interaction data sets. The high confidence in vivo protein data sets presented here add new data to the currently incomplete D. melanogaster interactome. Additionally we report contaminant proteins that are persistent with affinity purifications irrespective of the tagged bait.
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