ArticleNucleic acids research2011
Monitoring the reversible B to A-like transition of DNA in eukaryotic cells using Fourier transform infrared spectroscopy.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 207 citations in OpenAlex.
- Breed classification of Lao People's Democratic Republic (Lao PDR) and Thai native chickens using synchrotron radiation-based Fourier transform infrared spectroscopy and genotyping by sequencing.Poultry science · 2026Article
- IR Map of the Human Cell.Analytical chemistry · 2026Article
- Article
- Application of Fourier Transform Infrared Spectroscopy for Structural Analysis of RNAs.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Characterization of Rhesus Macaque Embryonic Stem Cells in Primed and Naïve-like Cell States of Pluripotency Using Fourier Transform Infrared (FTIR) Microspectroscopy.International journal of molecular sciences · 2025Article
- Marker-independent vibrational spectroscopy imaging recognizes the hypoxia effect in the human brain endothelium.Scientific reports · 2025Article
- Adaptation of DNA to Protein Binding Revealed by Spectroscopy and Molecular Simulation.The journal of physical chemistry. B · 2025Article
- Cold-induced fibrosis and metabolic remodeling in the turtle (Trachemys scripta) ventricle.Acta physiologica (Oxford, England) · 2025Article
- Structural features of DNA and their potential contribution to blind mole rat (Nannospalax xanthodon) longevity.Biogerontology · 2025Article
- NAIRDB: a database of Fourier transform infrared (FTIR) data for nucleic acids.Nucleic acids research · 2025Article
- Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy.Journal of synchrotron radiation · 2025Article
- Coassembly of a Hybrid Synthetic-Biological Chitosan-Polymers · 2024Article
- Decoding myasthenia gravis: advanced diagnosis with infrared spectroscopy and machine learning.Scientific reports · 2024Article
- Seasonal environmental transitions and metabolic plasticity in a sea-ice alga from an individual cell perspective.Scientific reports · 2024Article
- Infrared Spectroscopy in Gynecological Oncology: A Comprehensive Review of Diagnostic Potentials and Challenges.International journal of molecular sciences · 2024Review
- Biomolecular profiles of Arctic sea-ice diatoms highlight the role of under-ice light in cellular energy allocation.ISME communications · 2024Article
- Assessment of A- to B- DNA Transitions Utilizing the Drude Polarizable Force Field.Journal of chemical theory and computation · 2023Article
- Article
- Cell Phase Identification in a Three-Dimensional Engineered Tumor Model by Infrared Spectroscopic Imaging.Analytical chemistry · 2023Article
- Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells-A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy.Biomedicines · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to detect DNA conformation in eukaryotic cells is of paramount importance in understanding how some cells retain functionality in response to environmental stress. It is anticipated that the B to A transition might play a role in resistance to DNA damage such as heat, desiccation and toxic damage. To this end, conformational detail about the molecular structure of DNA has been derived primarily from in vitro experiments on extracted or synthetic DNA. Here, we report that a B- to A-like DNA conformational change can occur in the nuclei of intact cells in response to dehydration. This transition is reversible upon rehydration in air-dried cells. By systematically monitoring the dehydration and rehydration of single and double-stranded DNA, RNA, extracted nuclei and three types of eukaryotic cells including chicken erythrocytes, mammalian lymphocytes and cancerous rodent fibroblasts using Fourier transform infrared (FTIR) spectroscopy, we unequivocally assign the important DNA conformation marker bands within these cells. We also demonstrate that by applying FTIR spectroscopy to hydrated samples, the DNA bands become sharper and more intense. This is anticipated to provide a methodology enabling differentiation of cancerous from non-cancerous cells based on the increased DNA content inherent to dysplastic and neoplastic tissue.
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