ArticleNucleic acids research2011
The activity of the histone chaperone yeast Asf1 in the assembly and disassembly of histone H3/H4-DNA complexes.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed.
- Unraveling the mechanism of action of the PepGAT peptide against Candida albicans through proteomics approaches.Scientific reports · 2026Article
- Replication-coupled inheritance of chromatin states.Cell insight · 2024Review
- Symmetric inheritance of parental histones governs epigenome maintenance and embryonic stem cell identity.Nature genetics · 2023Article
- Article
- The Role of Chromatin and Transcriptional Control in the Formation of Sexual Fruiting Bodies in Fungi.Microbiology and molecular biology reviews : MMBR · 2022Review
- Mechanisms of chromatin-based epigenetic inheritance.Science China. Life sciences · 2022Review
- Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling.Nature communications · 2022Article
- Reduce, Retain, Recycle: Mechanisms for Promoting Histone Protein Degradation versus Stability and Retention.Molecular and cellular biology · 2021Review
- Review
- Multifaceted Roles of ICP22/ORF63 Proteins in the Life Cycle of Human Herpesviruses.Frontiers in microbiology · 2021Review
- Interactions With Histone H3 & Tools to Study Them.Frontiers in cell and developmental biology · 2020Review
- The replisome guides nucleosome assembly during DNA replication.Cell & bioscience · 2020Review
- The HIRA histone chaperone complex subunit UBN1 harbors H3/H4- and DNA-binding activity.The Journal of biological chemistry · 2019Article
- The histone chaperoning pathway: from ribosome to nucleosome.Essays in biochemistry · 2019Review
- Modulation of nucleosomal DNA accessibility via charge-altering post-translational modifications in histone core.Epigenetics & chromatin · 2018Article
- The elongation factor Spn1 is a multi-functional chromatin binding protein.Nucleic acids research · 2018Article
- Article
- Histone chaperone networks shaping chromatin function.Nature reviews. Molecular cell biology · 2017Review
- Partially Assembled Nucleosome Structures at Atomic Detail.Biophysical journal · 2017Article
- sNASP and ASF1A function through both competitive and compatible modes of histone binding.Nucleic acids research · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The deposition of the histones H3/H4 onto DNA to give the tetrasome intermediate and the displacement of H3/H4 from DNA are thought to be the first and the last steps in nucleosome assembly and disassembly, respectively. Anti-silencing function 1 (Asf1) is a chaperone of the H3/H4 dimer that functions in both of these processes. However, little is known about the thermodynamics of chaperone-histone interactions or the direct role of Asf1 in the formation or disassembly of histone-DNA complexes. Here, we show that Saccharomyces cerevisiae Asf1 shields H3/H4 from unfavorable DNA interactions and aids the formation of favorable histone-DNA interactions through the formation of disomes. However, Asf1 was unable to disengage histones from DNA for tetrasomes formed with H3/H4 and strong nucleosome positioning DNA sequences or tetrasomes weakened by mutant (H3K56Q/H4) histones or non-positioning DNA sequences. Furthermore, Asf1 did not associate with preformed tetrasomes. These results are consistent with the measured affinity of Asf1 for H3/H4 dimers of 2.5 nM, which is weaker than the association of H3/H4 for DNA. These studies support a mechanism by which Asf1 aids H3/H4 deposition onto DNA but suggest that additional factors or post-translational modifications are required for Asf1 to remove H3/H4 from tetrasome intermediates in chromatin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.