Evidence map›Paper›PMID 21447484›Full record

ArticleJournal of lipid research2011

Lipase maturation factor 1 is required for endothelial lipase activity.

Osnat Ben-Zeev, Maryam Hosseini, Ching-Mei Lai, Nicole Ehrhardt, Howard Wong, Angelo B Cefalù, Davide Noto, Maurizio R Averna, Mark H Doolittle, Miklós Péterfy

RetractedOpen access · hybridAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Purification, cellular levels, and functional domains of lipase maturation factor 1.Biochemical and biophysical research communications · 2014
    Article
  9. Article
  10. Review
  11. Review
  12. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Osnat Ben-ZeevDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA.
Maryam HosseiniDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA; Medical Genetics Institute, Cedars-Sinai Medical Center, Los Angeles, CA.
Ching-Mei LaiDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA.
Nicole EhrhardtMedical Genetics Institute, Cedars-Sinai Medical Center, Los Angeles, CA.
Howard WongDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA.
Angelo B CefalùDepartment of Clinical Medicine and Emerging Diseases, University of Palermo, Palermo, Italy.
Davide NotoDepartment of Clinical Medicine and Emerging Diseases, University of Palermo, Palermo, Italy.
Maurizio R AvernaDepartment of Clinical Medicine and Emerging Diseases, University of Palermo, Palermo, Italy.
Mark H DoolittleDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA.
Miklós PéterfyDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA; Medical Genetics Institute, Cedars-Sinai Medical Center, Los Angeles, CA. Electronic address: David_McLaren@merck.com.
VA Greater Los Angeles Healthcare System · USUniversity of Palermo · ITCedars-Sinai Medical Center · USUniversity of California, Los Angeles · US

Funding

Systems genomics of metabolic syndrome traitsP01HL028481 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI REUE, KAREN · 1985 to 2019
$50.2M
NHLBI NIH HHS HL-028481NHLBI NIH HHS P01 HL028481
6 · The paper itself

Abstract

Lipase maturation factor 1 (Lmf1) is an endoplasmic reticulum (ER) membrane protein involved in the posttranslational folding and/or assembly of lipoprotein lipase (LPL) and hepatic lipase (HL) into active enzymes. Mutations in Lmf1 are associated with diminished LPL and HL activities ("combined lipase deficiency") and result in severe hypertriglyceridemia in mice as well as in human subjects. Here, we investigate whether endothelial lipase (EL) also requires Lmf1 to attain enzymatic activity. We demonstrate that cells harboring a (cld) loss-of-function mutation in the Lmf1 gene are unable to generate active EL, but they regain this capacity after reconstitution with the Lmf1 wild type. Furthermore, we show that cellular EL copurifies with Lmf1, indicating their physical interaction in the ER. Finally, we determined that post-heparin phospholipase activity in a patient with the LMF1(W464X) mutation is reduced by more than 95% compared with that in controls. Thus, our study indicates that EL is critically dependent on Lmf1 for its maturation in the ER and demonstrates that Lmf1 is a required factor for all three vascular lipases, LPL, HL, and EL.

Indexed as

Membrane ProteinsAnimalsChromatography, AffinityElectroporationEndoplasmic ReticulumFibroblastsHEK293 CellsHumansHypertriglyceridemiaLipaseLipoprotein LipaseMiceMutationPlasmidsTransfectionLipaseLIPC protein, humanLIPG protein, humanLipoprotein LipaseLMF1 protein, humanMembrane Proteins

Identifiers

PMID21447484
PMCPMC3090237
OpenAlexW2136632748

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.