ArticleInternational journal for parasitology2011
Schistosoma mansoni U6 gene promoter-driven short hairpin RNA induces RNA interference in human fibrosarcoma cells and schistosomules.
Article in International journal for parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Identification and validation of a Schistosoma japonicum U6 promoter.Parasites & vectors · 2017Article
- Transfection of Platyhelminthes.BioMed research international · 2015Review
- Pseudotyped murine leukemia virus for schistosome transgenesis: approaches, methods and perspectives.Transgenic research · 2014Article
- Polyethyleneimine mediated DNA transfection in schistosome parasites and regulation of the WNT signaling pathway by a dominant-negative SmMef2.PLoS neglected tropical diseases · 2013Article
- Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.Transgenic research · 2012Article
- Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells.Transgenic research · 2012Article
- Genetic manipulation of schistosomes--progress with integration competent vectors.Parasitology · 2012Review
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
- Adenovirus-mediated Aurora A shRNA driven by stathmin promoter suppressed tumor growth and enhanced paclitaxel chemotherapy sensitivity in human breast carcinoma cells.Cancer gene therapy · 2012Article
- Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.PLoS pathogens · 2012Article
- New frontiers in schistosoma genomics and transcriptomics.Journal of parasitology research · 2012Article
- A research agenda for helminth diseases of humans: basic research and enabling technologies to support control and elimination of helminthiases.PLoS neglected tropical diseases · 2012Review
- Transposon-mediated chromosomal integration of transgenes in the parasitic nematode Strongyloides ratti and establishment of stable transgenic lines.PLoS pathogens · 2012Article
- Schistosoma comparative genomics: integrating genome structure, parasite biology and anthelmintic discovery.Trends in parasitology · 2011Review
- Progress with schistosome transgenesis.Memorias do Instituto Oswaldo Cruz · 2011Review
- Genetic manipulation of Schistosoma haematobium, the neglected schistosome.PLoS neglected tropical diseases · 2011Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
RNA interference (RNAi) mediated by short hairpin-RNA (shRNA) expressing plasmids can induce specific and long-term knockdown of specific mRNAs in eukaryotic cells. To develop a vector-based RNAi model for Schistosoma mansoni, the schistosome U6 gene promoter was employed to drive expression of shRNA targeting reporter firefly luciferase. An upstream region of a U6 gene predicted to contain the promoter was amplified from genomic DNA of S. mansoni. A shRNA construct driven by the predicted U6 promoter targeting luciferase was assembled and cloned into plasmid pXL-Bac II, the construct termed pXL-BacII_SmU6-shLuc. Luciferase expression in transgenic fibrosarcoma HT-1080 cells was significantly reduced 96 h following transduction with plasmid pXL-BacII_SmU6-shLuc, which encodes luciferase mRNA-specific shRNA. In a similar fashion, schistosomules of S. mansoni were transformed with the SmU6-shLuc or control constructs. Firefly luciferase mRNA was introduced into transformed schistosomules after which luciferase activity was analyzed. Significantly less activity was present in schistosomules transfected with pXL-BacII_SmU6-shLuc compared with controls. The findings revealed that the putative S. mansoni U6 gene promoter of 270 bp in length was active in human cells and schistosomes. Given that the U6 gene promoter drove expression of shRNA from an episome, the findings also indicate the potential of this putative RNA polymerase III dependent promoter as a component regulatory element in vector-based RNAi for functional genomics of schistosomes.
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