ArticleThe Journal of membrane biology2011
Characterization of TRPM8-like channels activated by the cooling agent icilin in the macrophage cell line RAW 264.7.
Article in The Journal of membrane biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- TRP (transient receptor potential) ion channel family: structures, biological functions and therapeutic interventions for diseases.Signal transduction and targeted therapy · 2023Review
- Function of TRP channels in monocytes/macrophages.Frontiers in immunology · 2023Review
- The cation channel TRPM8 influences the differentiation and function of human monocytes.Journal of leukocyte biology · 2022Article
- "Immuno-Transient Receptor Potential Ion Channels": The Role in Monocyte- and Macrophage-Mediated Inflammatory Responses.Frontiers in immunology · 2018Review
- Functional Role of Transient Receptor Potential Channels in Immune Cells and Epithelia.Frontiers in immunology · 2018Review
- Transient receptor potential melastatin 8 ion channel in macrophages modulates colitis through a balance-shift in TNF-alpha and interleukin-10 production.Mucosal immunology · 2016Article
- What is the evidence for the role of TRP channels in inflammatory and immune cells?British journal of pharmacology · 2016Review
- TRPM8 on mucosal sensory nerves regulates colitogenic responses by innate immune cells via CGRP.Mucosal immunology · 2015Article
- Multiple nanosecond electric pulses increase the number but not the size of long-lived nanopores in the cell membrane.Biochimica et biophysica acta · 2015Article
- Danger- and pathogen-associated molecular patterns recognition by pattern-recognition receptors and ion channels of the transient receptor potential family triggers the inflammasome activation in immune cells and sensory neurons.Journal of neuroinflammation · 2015Review
- Effects of ibandronate sodium, a nitrogen-containing bisphosphonate, on intermediate-conductance calcium-activated potassium channels in osteoclast precursor cells (RAW 264.7).The Journal of membrane biology · 2015Article
- Transient receptor potential ion channels in primary sensory neurons as targets for novel analgesics.British journal of pharmacology · 2014Review
- The effects of magnetite (Fe₃O₄) nanoparticles on electroporation-induced inward currents in pituitary tumor (GH₃) cells and in RAW 264.7 macrophages.International journal of nanomedicine · 2012Article
- Macrophage function in atherosclerosis: potential roles of TRP channels.Channels (Austin, Tex.)Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Icilin is recognized as a chemical agonist of nociceptors and can activate TRPM8 channels. However, whether this agent has any effects on immune cells remains unknown. In this study, the effects of icilin on ion currents were investigated in RAW 264.7 murine macrophage-like cells. Icilin (1-100 μM) increased the amplitude of nonselective (NS) cation current (INS) in a concentration-dependent manner with an EC50 value of 8.6 μM. LaCl3 (100 μM) or capsazepine (30 μM) reversed icilin-induced INS; however, neither apamin (200 nM) nor iberiotoxin (200 nM) had any effects on it. In cell-attached configuration, when the electrode was filled with icilin (30 μM), a unique population of NS cation channels were activated with single-channel conductance of 158 pS. With the use of a long-lasting ramp pulse protocol, increasing icilin concentration produced a left shift in the activation curve of NS channels, with no change in the slope factor of the curve. The probability of channel opening enhanced by icilin was increased by either elevated extracellular Ca2+ or application of ionomycin (10 μM), while it was reduced by BAPTA-AM (10 μM). Icilin-stimulated activity is associated with an increase in mean open time and a reduction in mean closed time. Under current-clamp conditions, icilin caused membrane depolarization. Therefore, icilin interacts with the TRPM8-like channel to increase INS and depolarizes the membrane in these cells.
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