ArticlePloS one2011
The ITS1-5.8S-ITS2 sequence region in the Musaceae: structure, diversity and use in molecular phylogeny.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Banana Classification Using Sanger Sequencing of the Ribosomal DNA Internal Transcribed Spacer (ITS) Region.Plants (Basel, Switzerland) · 2024Article
- Identification of Zimbabwe's locally grown banana (Musa Spp.) cultivars using morphology and genome-targeted sequencing.Journal, genetic engineering & biotechnology · 2023Article
- The genomic and bulked segregant analysis ofaBIOTECH · 2022Article
- Assessment of genetic diversity and volatile content of commercially grown banana (Musa spp.) cultivars.Scientific reports · 2022Article
- Extensive nrDNA ITS polymorphism inFrontiers in plant science · 2022Article
- Variation in Ribosomal DNA in the GenusPlants (Basel, Switzerland) · 2021Article
- Unravelling the complex story of intergenomic recombination in ABB allotriploid bananas.Annals of botany · 2021Article
- Reassessing Banana Phylogeny and Organelle Inheritance Modes Using Genome Skimming Data.Frontiers in plant science · 2021Article
- Validation of a Triplex Quantitative Polymerase Chain Reaction Assay for Detection and Quantification of Traditional Protein Sources,Frontiers in plant science · 2021Article
- Chromosome Painting in Cultivated Bananas and Their Wild Relatives (International journal of molecular sciences · 2020Article
- Molecular identification and evolutionary relationships between the subspecies of Musa by DNA barcodes.BMC genomics · 2020Article
- RIP mutated ITS genes in populations ofIMA fungus · 2020Article
- Genetic polymorphism of Japanese cultivated Rheum species in the internal transcribed spacer region of nuclear ribosomal DNA.Journal of natural medicines · 2019Article
- Molecular and Cytogenetic Study of East African Highland Banana.Frontiers in plant science · 2018Article
- New specific primers for amplification of the Internal Transcribed Spacer region in Clitellata (Annelida).Ecology and evolution · 2017Article
- Dominant Allele Phylogeny and Constitutive Subgenome Haplotype Inference in Bananas Using Mitochondrial and Nuclear Markers.Genome biology and evolution · 2017Article
- Article
- ITS non-concerted evolution and rampant hybridization in the legume genus Lespedeza (Fabaceae).Scientific reports · 2017Article
- DArT whole genome profiling provides insights on the evolution and taxonomy of edible Banana (Musa spp.).Annals of botany · 2016Article
- Evolutionary dynamics and biogeography of Musaceae reveal a correlation between the diversification of the banana family and the geological and climatic history of Southeast Asia.The New phytologist · 2016Article
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6 authors.
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Abstract
Genes coding for 45S ribosomal RNA are organized in tandem arrays of up to several thousand copies and contain 18S, 5.8S and 26S rRNA units separated by internal transcribed spacers ITS1 and ITS2. While the rRNA units are evolutionary conserved, ITS show high level of interspecific divergence and have been used frequently in genetic diversity and phylogenetic studies. In this work we report on the structure and diversity of the ITS region in 87 representatives of the family Musaceae. We provide the first detailed information on ITS sequence diversity in the genus Musa and describe the presence of more than one type of ITS sequence within individual species. Both Sanger sequencing of amplified ITS regions and whole genome 454 sequencing lead to similar phylogenetic inferences. We show that it is necessary to identify putative pseudogenic ITS sequences, which may have negative effect on phylogenetic reconstruction at lower taxonomic levels. Phylogenetic reconstruction based on ITS sequence showed that the genus Musa is divided into two distinct clades--Callimusa and Australimusa and Eumusa and Rhodochlamys. Most of the intraspecific banana hybrids analyzed contain conserved parental ITS sequences, indicating incomplete concerted evolution of rDNA loci. Independent evolution of parental rDNA in hybrids enables determination of genomic constitution of hybrids using ITS. The observation of only one type of ITS sequence in some of the presumed interspecific hybrid clones warrants further study to confirm their hybrid origin and to unravel processes leading to evolution of their genomes.
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