ArticlePloS one2011
Linking genotype and phenotype of Saccharomyces cerevisiae strains reveals metabolic engineering targets and leads to triterpene hyper-producers.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 51 citations in OpenAlex.
- The Role of Plant-Derived Natural Products as a Regulator of the Tyrosine Kinase Pathway in the Management of Lung Cancer.Current issues in molecular biology · 2025Review
- Understanding adaptation to fluconazole: comparative insights into tolerance and resistance inFrontiers in cellular and infection microbiology · 2025Article
- Different transcriptomic and metabolomic analysis of Saccharomyces cerevisiae BY4742 and CEN.PK2-1C strains.Archives of microbiology · 2024Article
- High production of triterpenoids inBiotechnology for biofuels · 2020Article
- Anaerobic growth of Saccharomyces cerevisiae CEN.PK113-7D does not depend on synthesis or supplementation of unsaturated fatty acids.FEMS yeast research · 2019Article
- Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering.Microbial cell factories · 2019Article
- Whole-cell (+)-ambrein production in the yeastMetabolic engineering communications · 2018Article
- β-Amyrin synthase fromFEBS open bio · 2017Article
- The sole introduction of two single-point mutations establishes glycerol utilization inBiotechnology for biofuels · 2017Article
- Improvement of betulinic acid biosynthesis in yeast employing multiple strategies.BMC biotechnology · 2016Article
- Deciphering the signaling mechanisms of the plant cell wall degradation machinery in Aspergillus oryzae.BMC systems biology · 2015Article
- Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.Microbial cell factories · 2015Article
- Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16α hydroxylase from Bupleurum falcatum.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Producing aglycons of ginsenosides in bakers' yeast.Scientific reports · 2014Article
- Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae.Microbial cell factories · 2012Article
- Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries.Cellular and molecular life sciences : CMLS · 2012Review
- Whole-genome sequencing of the efficient industrial fuel-ethanol fermentative Saccharomyces cerevisiae strain CAT-1.Molecular genetics and genomics : MGG · 2012Article
- De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology.Microbial cell factories · 2012Article
- Developing a yeast cell factory for the production of terpenoids.Computational and structural biotechnology journal · 2012Review
- Analyzing the genomic variation of microbial cell factories in the era of "New Biotechnology".Computational and structural biotechnology journal · 2012Review
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Authors and funding
9 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetabolic engineering is an attractive approach in order to improve the microbial production of drugs. Triterpenes is a chemically diverse class of compounds and many among them are of interest from a human health perspective. A systematic experimental or computational survey of all feasible gene modifications to determine the genotype yielding the optimal triterpene production phenotype is a laborious and time-consuming process. METHODOLOGY/PRINCIPAL
findingsBased on the recent genome-wide sequencing of Saccharomyces cerevisiae CEN.PK 113-7D and its phenotypic differences with the S288C strain, we implemented a strategy for the construction of a β-amyrin production platform. The genes Erg8, Erg9 and HFA1 contained non-silent SNPs that were computationally analyzed to evaluate the changes that cause in the respective protein structures. Subsequently, Erg8, Erg9 and HFA1 were correlated with the increased levels of ergosterol and fatty acids in CEN.PK 113-7D and single, double, and triple gene over-expression strains were constructed.
conclusionsThe six out of seven gene over-expression constructs had a considerable impact on both ergosterol and β-amyrin production. In the case of β-amyrin formation the triple over-expression construct exhibited a nearly 500% increase over the control strain making our metabolic engineering strategy the most successful design of triterpene microbial producers.
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