Evidence map›Paper›PMID 21445053›Full record

ArticleJournal of visualized experiments : JoVE2011

Preparation and culture of chicken auditory brainstem slices.

Jason T Sanchez, Armin H Seidl, Edwin W Rubel, Andres Barria

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
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  4. Article
  5. Article
  6. In Ovo Electroporation in the Chicken Auditory Brainstem.Journal of visualized experiments : JoVE · 2017
    Article
  7. Article
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jason T SanchezDepartment of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center, University of Washington, USA.
Armin H Seidl
Edwin W Rubel
Andres Barria

Funding

Developmental profile of glutamate receptors in nucleus laminarisF32DC010307 · NIDCD · UNIVERSITY OF WASHINGTON · PI SANCHEZ, JASON TAIT · 2009 to 2011
$157k
NIDCD NIH HHS F32 DC010307
6 · The paper itself

Abstract

The chicken auditory brainstem is a well-established model system that has been widely used to study the anatomy and physiology of auditory processing at discreet periods of development as well as mechanisms for temporal coding in the central nervous system. Here we present a method to prepare chicken auditory brainstem slices that can be used for acute experimental procedures or to culture organotypic slices for long-term experimental manipulations. The chicken auditory brainstem is composed of nucleus angularis, magnocellularis, laminaris and superior olive. These nuclei are responsible for binaural sound processing and single coronal slice preparations preserve the entire circuitry. Ultimately, organotypic slice cultures can provide the opportunity to manipulate several developmental parameters such as synaptic activity, expression of pre and postsynaptic components, expression of aspects controlling excitability and differential gene expression This approach can be used to broaden general knowledge about neural circuit development, refinement and maturation.

Indexed as

AnimalsBrain StemChick EmbryoCochlear NucleusMicrotomyOrgan Culture Techniques

Identifiers

PMID21445053
PMCPMC3197304

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.