ArticleMolecular and cellular biology2011
A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- H2A.Z deposition by the SWR complex is stimulated by polyadenine DNA sequences in nucleosomes.PLoS biology · 2025Article
- The Yeast HMGB Protein Hmo1 Is a Multifaceted Regulator of DNA Damage Tolerance.International journal of molecular sciences · 2025Article
- Exploring the Molecular Underpinnings of Cancer-Causing Oncohistone Mutants Using Yeast as a Model.Journal of fungi (Basel, Switzerland) · 2023Review
- Cryo-electron microscopy structure of the H3-H4 octasome: A nucleosome-like particle without histones H2A and H2B.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The role of gene dosage in budding yeast centrosome scaling and spontaneous diploidization.PLoS genetics · 2020Article
- Genome protection: histone H4 and beyond.Current genetics · 2020Review
- Systematic genetic and proteomic screens during gametogenesis identify H2BK34 methylation as an evolutionary conserved meiotic mark.Epigenetics & chromatin · 2020Article
- Cancer-associated mutations of histones H2B, H3.1 and H2A.Z.1 affect the structure and stability of the nucleosome.Nucleic acids research · 2018Article
- Dissecting Nucleosome Function with a Comprehensive Histone H2A and H2B Mutant Library.G3 (Bethesda, Md.) · 2017Article
- Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution.Microbiology spectrum · 2017Review
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination.Molecular biology of the cell · 2015Article
- Article
- Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment.Nucleic acids research · 2014Article
- Kinetochore function and chromosome segregation rely on critical residues in histones H3 and H4 in budding yeast.Genetics · 2013Article
- Structural integrity of centromeric chromatin and faithful chromosome segregation requires Pat1.Genetics · 2013Article
- The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres.Genes & development · 2012Article
- The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability.Epigenetics & chromatin · 2012Article
- Global analysis of core histones reveals nucleosomal surfaces required for chromosome bi-orientation.The EMBO journal · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A screen of Saccharomyces cerevisiae histone alanine substitution mutants revealed that mutations in any of three adjacent residues, L97, Y98, or G99, near the C terminus of H4 led to a unique phenotype. The mutants grew slowly, became polyploid or aneuploid rapidly, and also lost chromosomes at a high rate, most likely because their kinetochores were not assembled properly. There was lower histone occupancy, not only in the centromeric region, but also throughout the genome for the H4 mutants. The mutants displayed genetic interactions with the genes encoding two different histone chaperones, Rtt106 and CAF-I. Affinity purification of Rtt106 and CAF-I from yeast showed that much more H4 and H3 were bound to these histone chaperones in the case of the H4 mutants than in the wild type. However, in vitro binding experiments showed that the H4 mutant proteins bound somewhat more weakly to Rtt106 than did wild-type H4. These data suggest that the H4 mutant proteins, along with H3, accumulate on Rtt106 and CAF-I in vivo because they cannot be deposited efficiently on DNA or passed on to the next step in the histone deposition pathway, and this contributes to the observed genome instability and growth defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.