ArticleInfection and immunity2011
Deletion of mtrC in Haemophilus ducreyi increases sensitivity to human antimicrobial peptides and activates the CpxRA regulon.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Cathelicidins: Opportunities and Challenges in Skin Therapeutics and Clinical Translation.Antibiotics (Basel, Switzerland) · 2024Review
- The role of bacterial transport systems in the removal of host antimicrobial peptides in Gram-negative bacteria.FEMS microbiology reviews · 2022Review
- The Potential of Human Peptide LL-37 as an Antimicrobial and Anti-Biofilm Agent.Antibiotics (Basel, Switzerland) · 2021Review
- Interactions of the Skin PathogenFrontiers in immunology · 2020Review
- Mechanisms of sexually transmitted infection-induced inflammation in women: implications for HIV risk.Journal of the International AIDS Society · 2019Article
- Impaired Efflux of the Siderophore Enterobactin Induces Envelope Stress inFrontiers in microbiology · 2019Article
- Both MisR (CpxR) and MisS (CpxA) Are Required for Neisseria gonorrhoeae Infection in a Murine Model of Lower Genital Tract Infection.Infection and immunity · 2017Article
- ThemBio · 2017Article
- The SapA Protein Is Involved in Resistance to Antimicrobial Peptide PR-39 and Virulence ofFrontiers in microbiology · 2017Article
- Bacterial Evasion of Host Antimicrobial Peptide Defenses.Microbiology spectrum · 2016Review
- On the in vivo significance of bacterial resistance to antimicrobial peptides.Biochimica et biophysica acta · 2015Review
- Development and validation of a high-throughput cell-based screen to identify activators of a bacterial two-component signal transduction system.Antimicrobial agents and chemotherapy · 2015Article
- Phosphoethanolamine Transferase LptA in Haemophilus ducreyi Modifies Lipid A and Contributes to Human Defensin Resistance In Vitro.PloS one · 2015Article
- Host Antimicrobial Peptides in Bacterial Homeostasis and Pathogenesis of Disease.Antibiotics (Basel, Switzerland) · 2014Article
- Reciprocal regulation of resistance-nodulation-division efflux systems and the Cpx two-component system in Vibrio cholerae.Infection and immunity · 2014Article
- The Haemophilus ducreyi Fis protein is involved in controlling expression of the lspB-lspA2 operon and other virulence factors.Infection and immunity · 2013Article
- Activation of CpxRA in Haemophilus ducreyi primarily inhibits the expression of its targets, including major virulence determinants.Journal of bacteriology · 2013Article
- Permeases of the sap transporter are required for cathelicidin resistance and virulence of Haemophilus ducreyi in humans.The Journal of infectious diseases · 2012Article
- Sap transporter mediated import and subsequent degradation of antimicrobial peptides in Haemophilus.PLoS pathogens · 2011Article
- Rapid divergence of two classes of Haemophilus ducreyi.Journal of bacteriology · 2011Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Haemophilus ducreyi resists killing by antimicrobial peptides encountered during human infection, including cathelicidin LL-37, α-defensins, and β-defensins. In this study, we examined the role of the proton motive force-dependent multiple transferable resistance (MTR) transporter in antimicrobial peptide resistance in H. ducreyi. We found a proton motive force-dependent effect on H. ducreyi's resistance to LL-37 and β-defensin HBD-3, but not α-defensin HNP-2. Deletion of the membrane fusion protein MtrC rendered H. ducreyi more sensitive to LL-37 and human β-defensins but had relatively little effect on α-defensin resistance. The mtrC mutant 35000HPmtrC exhibited phenotypic changes in outer membrane protein profiles, colony morphology, and serum sensitivity, which were restored to wild type by trans-complementation with mtrC. Similar phenotypes were reported in a cpxA mutant; activation of the two-component CpxRA regulator was confirmed by showing transcriptional effects on CpxRA-regulated genes in 35000HPmtrC. A cpxR mutant had wild-type levels of antimicrobial peptide resistance; a cpxA mutation had little effect on defensin resistance but led to increased sensitivity to LL-37. 35000HPmtrC was more sensitive than the cpxA mutant to LL-37, indicating that MTR contributed to LL-37 resistance independent of the CpxRA regulon. The CpxRA regulon did not affect proton motive force-dependent antimicrobial peptide resistance; however, 35000HPmtrC had lost proton motive force-dependent peptide resistance, suggesting that the MTR transporter promotes proton motive force-dependent resistance to LL-37 and human β-defensins. This is the first report of a β-defensin resistance mechanism in H. ducreyi and shows that LL-37 resistance in H. ducreyi is multifactorial.
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