ArticleMethods (San Diego, Calif.)2011
Nucleosomal H2B ubiquitylation with purified factors.
Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- A systematic interactome ofeLife · 2026Article
- Restoration of retinal regenerative potential of Müller glia by disrupting intercellular Prox1 transfer.Nature communications · 2025Article
- PRMT1-mediated methylation regulates MLL2 stability and gene expression.Nucleic acids research · 2025Article
- Molecular basis for SOX2-dependent regulation of super-enhancer activity.Nucleic acids research · 2023Article
- Arginine 65 methylation of Neurogenin 3 by PRMT1 is required for pancreatic endocrine development of hESCs.Experimental & molecular medicine · 2023Article
- SHP-1 dephosphorylates histone H2B to facilitate its ubiquitination during transcription.The EMBO journal · 2022Article
- Article
- ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.Nucleic acids research · 2022Article
- H2B ubiquitylation enhances H3K4 methylation activities of human KMT2 family complexes.Nucleic acids research · 2020Article
- The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS.Nature communications · 2020Article
- RNF20/40-mediated eEF1BδL monoubiquitylation stimulates transcription of heat shock-responsive genes.Nucleic acids research · 2019Article
- Crosstalk among Set1 complex subunits involved in H2B ubiquitylation-dependent H3K4 methylation.Nucleic acids research · 2018Article
- Spinal RNF20-Mediated Histone H2B Monoubiquitylation Regulates mGluR5 Transcription for Neuropathic Allodynia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- MSK1 functions as a transcriptional coactivator of p53 in the regulation of p21 gene expression.Experimental & molecular medicine · 2018Article
- Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants.Nature communications · 2018Article
- Interactome Analysis Reveals a Novel Role for RAD6 in the Regulation of Proteasome Activity and Localization in Response to DNA Damage.Molecular and cellular biology · 2017Article
- Preparation and analysis of positioned mononucleosomes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Generation of nonhydrolyzable ubiquitin-histone mimics.Methods (San Diego, Calif.) · 2014Article
- Article
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2 authors at 1 institution in 1 country.
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Abstract
Diverse histone modifications play important roles in transcriptional regulation throughout eukaryotes, and recent studies have implicated histone H2B ubiquitylation in active transcription. The necessity of at least three enzymes (E1-E3), as well as ongoing transcription events, for efficient H2B ubiquitylation complicates mechanistic studies of H2B ubiquitylation relative to other histone modifications. Here we describe experimental protocols for preparation of human H2B ubiquitylation factors, ubiquitylation substrates and transcription factors, as well as the use of these factors to establish H2B ubiquitylation mechanisms during transcription. The methods include reliable protein interaction and E3 ubiquitylation assays that can be widely applied to confirm cognate E2-E3 pairs in other protein ubiquitylation systems, optimized in vitro ubiquitylation assays for various histone substrates, and a transcription-coupled H2B ubiquitylation assay in a highly purified transcription system. These comprehensive analyses have revealed (i) that RAD6 serves as the cognate E2 for the BRE1 complex in human cells, as previously established in yeast, (ii) that RAD6, through direct interaction with the BRE1 complex, ubiquitylates chromatinized H2B at lysine 120 and (iii) that PAF1 complex-mediated transcription is required for efficient H2B ubiquitylation. This experimental system permits detailed mechanistic analyses of H2B ubiquitylation during transcription by providing information concerning both precise enzyme functions and physical interactions between the transcription and histone modification machineries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.