ArticleMicrobial pathogenesis
Cytotoxic effects of Kingella kingae outer membrane vesicles on human cells.
Article in Microbial pathogenesis. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 61 citations in OpenAlex.
- PilY proteins: bimodular drivers of type IV pilus versatility.Trends in microbiology · 2026Review
- Fastidious or rarely isolated gram-negative rods with a particular focus onClinical microbiology reviews · 2025Review
- Differential Proteomic Analysis of Extracellular Vesicles Produced byDentistry journal · 2025Article
- The effect of site-specific recombinases XerCD on the removal of over-replicated chromosomal DNA through outer membrane vesicles in bacteria.Microbiology spectrum · 2024Article
- Prokaryotic microvesicles Ortholog of eukaryotic extracellular vesicles in biomedical fields.Cell communication and signaling : CCS · 2024Review
- Infective Endocarditis Due toMicroorganisms · 2024Review
- Immunomodulatory properties ofFrontiers in immunology · 2024Article
- Acquisition, co-option, and duplication of the rtx toxin system and the emergence of virulence in Kingella.Nature communications · 2023Article
- Role ofFrontiers in microbiology · 2023Review
- Roles of bacterial extracellular vesicles in systemic diseases.Frontiers in microbiology · 2023Review
- Outer Membrane Vesicles: Biogenesis, Functions, and Issues.Microbiology and molecular biology reviews : MMBR · 2022Review
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- Review
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- Pathogenic determinants ofFrontiers in pediatrics · 2022Review
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- Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity.Journal of visualized experiments : JoVE · 2021Article
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- Bacterial Outer Membrane Vesicles as Antibiotic Delivery Vehicles.Frontiers in immunology · 2021Review
- Proinflammatory Microenvironment DuringFrontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Kingella kingae is an emerging pathogen causing osteoarticular infections in pediatric patients. Electron microscopy of K. kingae clinical isolates revealed the heterogeneously-sized membranous structures blebbing from the outer membrane that were classified as outer membrane vesicles (OMVs). OMVs purified from the secreted fraction of a septic arthritis K. kingae isolate were characterized. Among several major proteins, K. kingae OMVs contained virulence factors RtxA toxin and PilC2 pilus adhesin. RtxA was also found secreted as a soluble protein in the extracellular environment indicating that the bacterium may utilize different mechanisms for the toxin delivery. OMVs were shown to be hemolytic and possess some leukotoxic activity while high leukotoxicity was detected in the non-hemolytic OMV-free component of the secreted fraction. OMVs were internalized by human osteoblasts and synovial cells. Upon interaction with OMVs, the cells produced increased levels of human granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 6 (IL-6) suggesting that these cytokines might be involved in the signaling response of infected joint and bone tissues during natural K. kingae infection. This study is the first report of OMV production by K. kingae and demonstrates that OMVs are a complex virulence factor of the organism causing cytolytic and inflammatory effects on host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.