ArticleBMC research notes2011
An RNA isolation system for plant tissues rich in secondary metabolites.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
125 citing papers in PubMed, 309 citations in OpenAlex.
- Genome-Wide Identification and Characterization of Thaumatin-Like Proteins in Potato (Solanum tuberosum L.) and Their Role in Stress Tolerance.Molecular biotechnology · 2026Article
- Metabolomic and transcriptomic analyses reveal the monoterpenoid indole alkaloid biosynthesis in Neolamarckia cadamba.Molecular biology reports · 2026Article
- Comprehensive genome-wide analysis of DUF668 gene family in potato reveals roles in growth and stress responses.Journal of applied genetics · 2026Article
- Unveiling the carbohydrate-active enzyme repertoire of Trichoderma caribbaeum IHBT through whole-genome analysis and transcriptional profiling.Archives of microbiology · 2026Article
- Comparative genome-wide analysis in Setaria italica and Arabidopsis thaliana revealed functional conservation of specific FtsH proteases under high light and heat stress.Plant cell reports · 2026Article
- Virome analysis of mango reveals mangifera indica latent virus, a benyvirus and a novel olivavirus.Virology journal · 2025Article
- Multi-omics profiling of Curcuma Wenyujin under salt-alkali stress reveals functional genes and associated metabolites.BMC plant biology · 2025Article
- Organ-specific transcriptional and untargeted metabolome analysis demystifies molecular insights and regulation of Gallic acid and flavonoid biosynthesis in Bergenia ciliata.Functional & integrative genomics · 2025Article
- Exploring the potential of Moringa oleifera seed extract-loaded microemulsion hydrogel in the DNCB-induced atopic dermatitis model.Inflammopharmacology · 2025Article
- Unveiling transcriptional regulation underpinning chilling and frost stress response in Trans-Himalayan Hippophae tibetana L.Plant molecular biology · 2025Article
- Effect of fieldwork-friendly coffee blender-based extraction methods and leaf tissue storage on the transcriptome of non-model plants.Journal of plant research · 2025Article
- High-quality haplotype-resolved chromosome assembly provides evolutionary insights and targeted steviol glycosides (SGs) biosynthesis in Stevia rebaudiana Bertoni.Plant biotechnology journal · 2024Article
- MYB transcription factors, their regulation and interactions with non-coding RNAs during drought stress in Brassica juncea.BMC plant biology · 2024Article
- Quantitative and spatially resolved detection of multiplexed microRNA from plant tissue via hybridization to hydrogel-bound DNA probes in nanoliter well arrays.Microsystems & nanoengineering · 2024Article
- CTAB Protocol for Obtaining High-Quality Total RNA fromACS omega · 2024Article
- A modified CTAB method for the extraction of high-quality RNA from mono-and dicotyledonous plants rich in secondary metabolites.Plant methods · 2024Article
- An Optimized and Cost-Effective RNA Extraction Method for Secondary Metabolite-Enriched Tissues of Norway Spruce (Plants (Basel, Switzerland) · 2024Article
- Article
- Quality control on oil palm RNA samples for efficient genomic downstream applications.Journal of genomics · 2024Article
- Review
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSecondary metabolites are reported to interfere with the isolation of RNA particularly with the recipes that use guanidinium-based salt. Such interference was observed in isolation of RNA with medicinal plants rheum (Rheum australe) and arnebia (Arnebia euchroma). A rapid and less cumbersome system for isolation of RNA was essential to facilitate any study related to gene expression.
findingsAn RNA isolation system free of guanidinium salt was developed that successfully isolated RNA from rheum and arnebia. The method took about 45 min and was successfully evaluated on twenty one tissues with varied secondary metabolites. The A260/280 ratio ranged between 1.8 - 2.0 with distinct 28 S and 18 S rRNA bands visible on a formaldehyde-agarose gel.
conclusionsThe present manuscript describes a rapid protocol for isolation of RNA, which works well with all the tissues examined so far. The remarkable feature was the success in isolation of RNA with those tissues, wherein the most commonly used methods failed. Isolated RNA was amenable to downstream applications such as reverse transcription-polymerase chain reaction (RT-PCR), differential display (DD), suppression subtractive hybridization (SSH) library construction, and northern hybridization.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.