ArticleBMC genomics2011
A cell spot microarray method for production of high density siRNA transfection microarrays.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening.High-throughput · 2018Article
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
- Living Cell Microarrays: An Overview of Concepts.Microarrays (Basel, Switzerland) · 2016Review
- Oncogenic Herpesvirus Utilizes Stress-Induced Cell Cycle Checkpoints for Efficient Lytic Replication.PLoS pathogens · 2016Article
- Protein Microarrays with Novel Microfluidic Methods: Current Advances.Microarrays (Basel, Switzerland) · 2014Review
- Molecular deconstruction, detection, and computational prediction of microenvironment-modulated cellular responses to cancer therapeutics.Advanced drug delivery reviews · 2014Review
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- Functional screening identifies miRNAs influencing apoptosis and proliferation in colorectal cancer.PloS one · 2014Article
- CDK-mediated activation of the SCF(FBXO) (28) ubiquitin ligase promotes MYC-driven transcription and tumourigenesis and predicts poor survival in breast cancer.EMBO molecular medicine · 2013Article
- Quantitative genetic-interaction mapping in mammalian cells.Nature methods · 2013Article
- Expanding the Diversity of Imaging-Based RNAi Screen Applications Using Cell Spot Microarrays.Microarrays (Basel, Switzerland) · 2013Article
- MiR-9, -31, and -182 deregulation promote proliferation and tumor cell survival in colon cancer.Neoplasia (New York, N.Y.) · 2012Article
- The gene expression landscape of breast cancer is shaped by tumor protein p53 status and epithelial-mesenchymal transition.Breast cancer research : BCR · 2012Article
- Article
- Optimizing cell arrays for accurate functional genomics.BMC research notes · 2012Article
- MicroSCALE screening reveals genetic modifiers of therapeutic response in melanoma.Science signaling · 2012Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh-throughput RNAi screening is widely applied in biological research, but remains expensive, infrastructure-intensive and conversion of many assays to HTS applications in microplate format is not feasible.
resultsHere, we describe the optimization of a miniaturized cell spot microarray (CSMA) method, which facilitates utilization of the transfection microarray technique for disparate RNAi analyses. To promote rapid adaptation of the method, the concept has been tested with a panel of 92 adherent cell types, including primary human cells. We demonstrate the method in the systematic screening of 492 GPCR coding genes for impact on growth and survival of cultured human prostate cancer cells.
conclusionsThe CSMA method facilitates reproducible preparation of highly parallel cell microarrays for large-scale gene knockdown analyses. This will be critical towards expanding the cell based functional genetic screens to include more RNAi constructs, allow combinatorial RNAi analyses, multi-parametric phenotypic readouts or comparative analysis of many different cell types.
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