ArticleInternational orthopaedics2012
Does multiplex polymerase chain reaction increase the diagnostic percentage in osteoarticular tuberculosis? A prospective evaluation of 80 cases.
Article in International orthopaedics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Tuberculosis of the spine.World journal of orthopedics · 2023Review
- Article
- Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis.Clinical rheumatology · 2021Article
- Review
- Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis.JGH open : an open access journal of gastroenterology and hepatology · 2019Article
- Comparative Study of GeneXpert MTB/RIF Assay and Multiplex PCR Assay for Direct Detection of Mycobacterium tuberculosis in Suspected Pulmonary Tuberculosis Patients.Current microbiology · 2017Article
- Article
- Role of alpha-crystallin, early-secreted antigenic target 6-kDa protein and culture filtrate protein 10 as novel diagnostic markers in osteoarticular tuberculosis.Journal of orthopaedic translation · 2016Review
- Performance of a pyrosequencing platform in diagnosing drug-resistant extra-pulmonary tuberculosis in India.The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2016Article
- Comparative Evaluation of Several Gene Targets for Designing a Multiplex-PCR for an Early Diagnosis of Extrapulmonary Tuberculosis.Yonsei medical journal · 2016Article
- Utility of adenosine deaminase (ADA), PCR & thoracoscopy in differentiating tuberculous & non-tuberculous pleural effusion complicating chronic kidney disease.The Indian journal of medical research · 2015Article
- Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis.Journal of global infectious diseases · 2013Article
- Novel multi-targeted polymerase chain reaction for diagnosis of presumed tubercular uveitis.Journal of ophthalmic inflammation and infection · 2013Article
- Infections of the spine are still a great problem.International orthopaedics · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMultiplex Polymerase Chain Reaction (MPCR) is a technique in which two or more gene targets are amplified in a single reaction. This has increased sensitivity of diagnosis as a single gene target may be absent in some Mycobacterium tuberculosis strains.
methodsMPCR using two target genes specific for Mycobacterium tuberculosis, that is, IS6110 and MPB 64, ZN staining and Mycobacterial culture were performed on synovial fluid/pus samples of 80 (three confirmed, 77 suspected) patients of osteoarticular tuberculosis and 25 non tuberculosis patients.
resultsMPCR had a sensitivity of 100% in confirmed cases and 81.8% in clinically suspected cases. AFB was positive in one patient and Mycobacterial culture was positive in three patients. MPCR also had 100% specificity; MPB64 was positive in five patients in which IS6110 was negative whereas IS6110 was positive in two patients in which MPB64 was negative.
conclusionsMPCR is a sensitive and specific method for diagnosis of paucibacilliary conditions such as osteoarticular tuberculosis.
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