ArticleJournal of bacteriology2011
The phytopathogen Pseudomonas syringae pv. tomato DC3000 has three high-affinity iron-scavenging systems functional under iron limitation conditions but dispensable for pathogenesis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 88 citations in OpenAlex.
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- The Role of Iron in Phytopathogenic Microbe-Plant Interactions: Insights into Virulence and Host Immune Response.Plants (Basel, Switzerland) · 2023Review
- Biophysical and proteomic analyses ofmBio · 2023Article
- The fciTABC and feoABI systems contribute to ferric citrate acquisition in Stenotrophomonas maltophilia.Journal of biomedical science · 2022Article
- Shared in planta population and transcriptomic features of nonpathogenic members of endophytic phyllosphere microbiota.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Negatively Regulated Aerobactin and Desferrioxamine E by Fur in Pantoea ananatis Are Required for Full Siderophore Production and Antibacterial Activity, but Not for Virulence.Applied and environmental microbiology · 2022Article
- Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato, and Barley.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genome-guided comparativeFrontiers in plant science · 2022Article
- The Knockout of Enterobactin-Related Gene inInternational journal of molecular sciences · 2021Article
- Bacterial effector targeting of a plant iron sensor facilitates iron acquisition and pathogen colonization.The Plant cell · 2021Article
- HrpL Regulon of Bacterial Pathogen of Woody HostMicroorganisms · 2021Article
- Iron homeostasis and plant immune responses: Recent insights and translational implications.The Journal of biological chemistry · 2020Review
- Re-evaluation of a Tn5::gacA mutant of Pseudomonas syringae pv. tomato DC3000 uncovers roles for uvrC and anmK in promoting virulence.PloS one · 2019Article
- Community-led comparative genomic and phenotypic analysis of the aquaculture pathogen Pseudomonas baetica a390T sequenced by Ion semiconductor and Nanopore technologies.FEMS microbiology letters · 2018Article
- Transcriptome landscape of a bacterial pathogen under plant immunity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Xanthoferrin, the α-hydroxycarboxylate-type siderophore of Xanthomonas campestris pv. campestris, is required for optimum virulence and growth inside cabbage.Molecular plant pathology · 2017Article
- Comparative Genome Analysis ofThe plant pathology journal · 2017Article
- Secreted Citrate Serves as Iron Carrier for the Marine PathogenFrontiers in cellular and infection microbiology · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-affinity iron scavenging through the use of siderophores is a well-established virulence determinant in mammalian pathogenesis. However, few examples have been reported for plant pathogens. Here, we use a genetic approach to investigate the role of siderophores in Pseudomonas syringae pv. tomato DC3000 (DC3000) virulence in tomato. DC3000, an agronomically important pathogen, has two known siderophores for high-affinity iron scavenging, yersiniabactin and pyoverdin, and we uncover a third siderophore, citrate, required for growth when iron is limiting. Though growth of a DC3000 triple mutant unable to either synthesize or import these siderophores is severely restricted in iron-limited culture, it is fully pathogenic. One explanation for this phenotype is that the DC3000 triple mutant is able to directly pirate plant iron compounds such as heme/hemin or iron-nicotianamine, and our data indicate that DC3000 can import iron-nicotianamine with high affinity. However, an alternative explanation, supported by data from others, is that the pathogenic environment of DC3000 (i.e., leaf apoplast) is not iron limited but is iron replete, with available iron of >1 μM. Growth of the triple mutant in culture is restored to wild-type levels by supplementation with a variety of iron chelates at >1 μM, including iron(III) dicitrate, a dominant chelate of the leaf apoplast. This suggests that lower-affinity iron import would be sufficient for DC3000 iron nutrition in planta and is in sharp contrast to the high-affinity iron-scavenging mechanisms required in mammalian pathogenesis.
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