ArticleApplied and environmental microbiology2011
Production of N-acetyl-D-neuraminic acid by use of an efficient spore surface display system.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Highly sensitive protein detection using recombinant spores and lateral flow immunoassay.Analytical and bioanalytical chemistry · 2021Article
- Surface display of p75, a Lactobacillus rhamnosus GG derived protein, on Bacillus subtilis spores and its antibacterial activity against Listeria monocytogenes.AMB Express · 2020Article
- Progress in research and application development of surface display technology using Bacillus subtilis spores.Applied microbiology and biotechnology · 2020Review
- Bacillus subtilis Spore Surface Display of Haloalkane Dehalogenase DhaA.Current microbiology · 2019Article
- Molecular Characterization of a Novel N-Acetylneuraminate Lyase from a Deep-Sea Symbiotic Mycoplasma.Marine drugs · 2018Article
- A self-sufficient system for removal of synthetic dye by coupling of spore-displayed triphenylmethane reductase and glucose 1-dehydrogenase.Environmental science and pollution research international · 2016Article
- Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein.Journal of industrial microbiology & biotechnology · 2015Article
- Surface display of the thermophilic lipase Tm1350 on the spore of Bacillus subtilis by the CotB anchor protein.Extremophiles : life under extreme conditions · 2015Article
- Engineering Novel and Improved Biocatalysts by Cell Surface Display.Industrial & engineering chemistry research · 2015Article
- Characterization of a novel N-acetylneuraminic acid lyase favoring industrial N-acetylneuraminic acid synthesis.Scientific reports · 2015Article
- In vivo self-assembly of stable green fluorescent protein fusion particles and their uses in enzyme immobilization.Applied and environmental microbiology · 2014Article
- Bioengineering of bacterial polymer inclusions catalyzing the synthesis of N-acetylneuraminic acid.Applied and environmental microbiology · 2013Article
- Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase.Applied and environmental microbiology · 2011Article
- One-pot bio-synthesis: N-acetyl-D-neuraminic acid production by a powerful engineered whole-cell catalyst.Scientific reports · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of N-acetyl-D-neuraminic acid (Neu5Ac) via biocatalysis is traditionally conducted using isolated enzymes or whole cells. The use of isolated enzymes is restricted by the time-consuming purification process, whereas the application of whole cells is limited by the permeability barrier presented by the microbial cell membrane. In this study, a novel type of biocatalyst, Neu5Ac aldolase presented on the surface of Bacillus subtilis spores, was used for the production of Neu5Ac. Under optimal conditions, Neu5Ac at a high concentration (54.7 g liter⁻¹) and a high yield (90.2%) was obtained under a 5-fold excess of pyruvate over N-acetyl-D-mannosamine. The novel biocatalyst system, which is able to express and immobilize the target enzyme simultaneously on the surface of B. subtilis spores, represents a suitable alternative for value-added chemical production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.