ArticleMolecular biology of the cell2011
Exophilin8 transiently clusters insulin granules at the actin-rich cell cortex prior to exocytosis.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Roles of molecular motors in insulin-secreting beta cells.Current opinion in cell biology · 2025Review
- Multiple pathways and independent functional pools in insulin granule exocytosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.eLife · 2023Article
- Coronin1C Is a GDP-Specific Rab44 Effector That Controls Osteoclast Formation by Regulating Cell Motility in Macrophages.International journal of molecular sciences · 2022Article
- Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion.Cell structure and function · 2022Article
- The changing view of insulin granule mobility: From conveyor belt to signaling hub.Frontiers in endocrinology · 2022Review
- In vivo Roles of Rab27 and Its Effectors in Exocytosis.Cell structure and function · 2021Review
- Targeting Small GTPases and Their Prenylation in Diabetes Mellitus.Journal of medicinal chemistry · 2021Review
- Article
- Granuphilin exclusively mediates functional granule docking to the plasma membrane.Scientific reports · 2016Article
- The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells.Scientific reports · 2015Article
- Interplay between Rab27a effectors in pancreatic β-cells.World journal of diabetes · 2015Review
- Cytoskeletal dependence of insulin granule movement dynamics in INS-1 beta-cells in response to glucose.PloS one · 2014Article
- Activated Cdc42-bound IQGAP1 determines the cellular endocytic site.Molecular and cellular biology · 2013Article
- Signaling mechanisms of glucose-induced F-actin remodeling in pancreatic islet β cells.Experimental & molecular medicine · 2013Review
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- The Rab27a effector exophilin7 promotes fusion of secretory granules that have not been docked to the plasma membrane.Molecular biology of the cell · 2013Article
- MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.Molecular biology of the cell · 2012Article
- Myrip couples the capture of secretory granules by the actin-rich cell cortex and their attachment to the plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.Traffic (Copenhagen, Denmark)Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exophilin8/MyRIP/Slac2-c is an effector protein of the small GTPase Rab27a and is specifically localized on retinal melanosomes and secretory granules. We investigated the role of exophilin8 in insulin granule trafficking. Exogenous expression of exophilin8 in pancreatic β cells or their cell line, MIN6, polarized (exophilin8-positive) insulin granules at the cell corners, where both cortical actin and the microtubule plus-end-binding protein, EB1, were present. Mutation analyses indicated that the ability of exophilin8 to act as a linker between Rab27a and myosin Va is essential for its granule-clustering activity. Moreover, exophilin8 and exophilin8-associated insulin granules were markedly stable and immobile. Total internal reflection fluorescence microscopy indicated that exophilin8 restricts the motion of insulin granules at a region deeper than that where another Rab27a effector, granuphilin, accumulates docked granules directly attached to the plasma membrane. However, the exophilin8-induced immobility of insulin granules was eliminated upon secretagogue stimulation and did not inhibit evoked exocytosis. Furthermore, exophilin8 depletion prevents insulin granules from being transported close to the plasma membrane and inhibits their fusion. These findings indicate that exophilin8 transiently traps insulin granules into the cortical actin network close to the microtubule plus-ends and supplies them for release during the stimulation.
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