ArticleGenetics2011
Circumventing heterozygosity: sequencing the amplified genome of a single haploid Drosophila melanogaster embryo.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Chromosome-level genome assembly and transcriptomes of the leaf insect Cryptophyllium westwoodii provide insights into the evolution of leaf-like masquerade.GigaScience · 2026Article
- Genetic variation in recalcitrant repetitive regions of theGenome research · 2025Article
- Article
- Genomic Diversity Illuminates the Environmental Adaptation of Drosophila suzukii.Genome biology and evolution · 2024Article
- Variation in fine-scale recombination rate in temperature-evolved Drosophila melanogaster populations in response to selection.G3 (Bethesda, Md.) · 2022Article
- Epistatic selection on a selfisheLife · 2022Article
- Detecting selected haplotype blocks in evolve and resequence experiments.Molecular ecology resources · 2021Article
- Genomic Responses to Climate Change: Making the Most of theFrontiers in genetics · 2021Article
- Demographic analyses of a new sample of haploid genomes from a Swedish population of Drosophila melanogaster.Scientific reports · 2020Article
- Population Genomics on the Fly: Recent Advances in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Population Genetic and Functional Analysis of aGenes · 2019Article
- Small Segmental Duplications in Drosophila-High Rate of Emergence and Elimination.Genome biology and evolution · 2019Article
- Gene Regulatory Variation inGenetics · 2018Article
- Charting the genotype-phenotype map: lessons from the Drosophila melanogaster Genetic Reference Panel.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Article
- A genome-wide scan for genes under balancing selection in Drosophila melanogaster.BMC evolutionary biology · 2017Article
- A Hidden Markov Model Approach for Simultaneously Estimating Local Ancestry and Admixture Time Using Next Generation Sequence Data in Samples of Arbitrary Ploidy.PLoS genetics · 2017Article
- A Thousand Fly Genomes: An Expanded Drosophila Genome Nexus.Molecular biology and evolution · 2016Article
- A haplotype method detects diverse scenarios of local adaptation from genomic sequence variation.Molecular ecology · 2016Article
- High rates of phasing errors in highly polymorphic species with low levels of linkage disequilibrium.Molecular ecology resources · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Heterozygosity is a major challenge to efficient, high-quality genomic assembly and to the full genomic survey of polymorphism and divergence. In Drosophila melanogaster lines derived from equatorial populations are particularly resistant to inbreeding, thus imposing a major barrier to the determination and analyses of genomic variation in natural populations of this model organism. Here we present a simple genome sequencing protocol based on the whole-genome amplification of the gynogenetically derived haploid genome of a progeny of females mated to males homozygous for the recessive male sterile mutation, ms(3)K81. A single "lane" of paired-end sequences (2 × 76 bp) provides a good syntenic assembly with >95% high-quality coverage (more than five reads). The amplification of the genomic DNA moderately inflates the variation in coverage across the euchromatic portion of the genome. It also increases the frequency of chimeric clones. But the low frequency and random genomic distribution of the chimeric clones limits their impact on the final assemblies. This method provides a solid path forward for population genomic sequencing and offers applications to many other systems in which small amounts of genomic DNA have unique experimental relevance.
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