ArticleGenetics2011
Nitrogen metabolite repression of metabolism and virulence in the human fungal pathogen Cryptococcus neoformans.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 81 citations in OpenAlex.
- Article
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- Isolation and Identification of Apiospora intestini from Hedera nepalensis Leaf Blight and Determination of Antagonism of Phomopsis sp.Current microbiology · 2025Article
- Unveiling new features of the human pathogenComputational and structural biotechnology journal · 2025Article
- Exposure of Cryptococcus neoformans to low nitrogen levels enhances virulence.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Transcriptomic investigation of the interaction between a biocontrol yeast, Papiliotrema terrestris strain PT22AV, and the postharvest fungal pathogen Penicillium expansum on apple.Communications biology · 2024Article
- Pleiotropic roles of LAMMER kinase, Lkh1 in stress responses and virulence ofFrontiers in cellular and infection microbiology · 2024Article
- A Limited Number of Amino Acid Permeases Are Crucial forInternational journal of microbiology · 2024Review
- Proteomics ofJournal of fungi (Basel, Switzerland) · 2023Article
- Factors Influencing the Nitrogen-Source Dependent Flucytosine Resistance in Cryptococcus Species.mBio · 2023Article
- The Combination of Iron and Copper Increases Pathogenicity and Induces Proteins Related to the Main Virulence Factors in Clinical Isolates ofJournal of fungi (Basel, Switzerland) · 2022Article
- Predicted Input of Uncultured Fungal Symbionts to a Lichen Symbiosis from Metagenome-Assembled Genomes.Genome biology and evolution · 2021Article
- A Conserved Gcn2-Gcn4 Axis Links Methionine Utilization and the Oxidative Stress Response inFrontiers in fungal biology · 2021Article
- A Transcriptional Regulatory Map of Iron Homeostasis Reveals a New Control Circuit for Capsule Formation inGenetics · 2020Article
- Article
- Defining Critical Genes During Spherule Remodeling and Endospore Development in the Fungal Pathogen,Frontiers in genetics · 2020Article
- Landscape of gene expression variation of natural isolates ofMicrobial genomics · 2020Article
- GATA-type transcription factors play a vital role in radiation sensitivity of Cryptococcus neoformans by regulating the gene expression of specific amino acid permeases.Scientific reports · 2019Article
- Draft Genome Sequence of Phoma arachidicola Wb2 Causing Peanut Web Blotch in China.Current microbiology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proper regulation of metabolism is essential to maximizing fitness of organisms in their chosen environmental niche. Nitrogen metabolite repression is an example of a regulatory mechanism in fungi that enables preferential utilization of easily assimilated nitrogen sources, such as ammonium, to conserve resources. Here we provide genetic, transcriptional, and phenotypic evidence of nitrogen metabolite repression in the human pathogen Cryptococcus neoformans. In addition to loss of transcriptional activation of catabolic enzyme-encoding genes of the uric acid and proline assimilation pathways in the presence of ammonium, nitrogen metabolite repression also regulates the production of the virulence determinants capsule and melanin. Since GATA transcription factors are known to play a key role in nitrogen metabolite repression, bioinformatic analyses of the C. neoformans genome were undertaken and seven predicted GATA-type genes were identified. A screen of these deletion mutants revealed GAT1, encoding the only global transcription factor essential for utilization of a wide range of nitrogen sources, including uric acid, urea, and creatinine-three predominant nitrogen constituents found in the C. neoformans ecological niche. In addition to its evolutionarily conserved role in mediating nitrogen metabolite repression and controlling the expression of catabolic enzyme and permease-encoding genes, Gat1 also negatively regulates virulence traits, including infectious basidiospore production, melanin formation, and growth at high body temperature (39°-40°). Conversely, Gat1 positively regulates capsule production. A murine inhalation model of cryptococcosis revealed that the gat1Δ mutant is slightly more virulent than wild type, indicating that Gat1 plays a complex regulatory role during infection.
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