ArticleCurrent biology : CB2011
Amphiastral mitotic spindle assembly in vertebrate cells lacking centrosomes.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- The histone H3.3 K27M mutation suppresses Ser31phosphorylation and mitotic fidelity, which can directly drive gliomagenesis.Current biology : CB · 2025Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Centrosomal and Non-centrosomal Functions Emerged through Eliminating Centrosomes.Cell structure and function · 2020Review
- NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells.The EMBO journal · 2020Article
- Manipulating cultured mammalian cells for mitosis research.Methods in cell biology · 2020Article
- Characterization of Novel Murine and Human PDAC Cell Models: Identifying the Role of Intestine Specific Homeobox Gene ISX in Hypoxia and Disease Progression.Translational oncology · 2019Article
- CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.Autophagy · 2018Article
- Mitotic spindle assembly in animal cells: a fine balancing act.Nature reviews. Molecular cell biology · 2017Review
- Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation.Nature cell biology · 2016Article
- Centrosomes are multifunctional regulators of genome stability.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Review
- Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo.PLoS genetics · 2015Article
- A novel strategy for targeted killing of tumor cells: Induction of multipolar acentrosomal mitotic spindles with a quinazolinone derivative mdivi-1.Molecular oncology · 2015Article
- Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.Nature genetics · 2014Article
- One to only two: a short history of the centrosome and its duplication.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Lack of centrioles and primary cilia in STIL(-/-) mouse embryos.Cell cycle (Georgetown, Tex.) · 2014Article
- Loss of centrioles causes chromosomal instability in vertebrate somatic cells.The Journal of cell biology · 2013Article
- Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Self-assembly and sorting of acentrosomal microtubules by TACC3 facilitate kinetochore capture during the mitotic spindle assembly.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Non-vesicular trafficking by a ceramide-1-phosphate transfer protein regulates eicosanoids.Nature · 2013Article
- Centrosome positioning in vertebrate development.Journal of cell science · 2012Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
The role of centrosomes and centrioles during mitotic spindle assembly in vertebrates remains controversial. In cell-free extracts and experimentally derived acentrosomal cells, randomly oriented microtubules (MTs) self-organize around mitotic chromosomes and assemble anastral spindles. However, vertebrate somatic cells normally assemble a connected pair of polarized, astral MT arrays--termed an amphiaster ("a star on both sides")--that is formed by the splitting and separation of the microtubule-organizing center (MTOC) well before nuclear envelope breakdown (NEB). Whether amphiaster formation requires splitting of duplicated centrosomes is not known. We found that when centrosomes were removed from living vertebrate cells early in their cell cycle, an acentriolar MTOC reassembled, and, prior to NEB, a functional amphiastral spindle formed. Cytoplasmic dynein, dynactin, and pericentrin are all recruited to the interphase aMTOC, and the activity of kinesin-5 is needed for amphiaster formation. Mitosis proceeded on time and these karyoplasts divided in two. However, ~35% of aMTOCs failed to split and separate before NEB, and these entered mitosis with persistent monastral spindles. Chromatin-associated RAN-GTP--the small GTPase Ran in its GTP bound state--could not restore bipolarity to monastral spindles, and these cells exited mitosis as single daughters. Our data reveal the novel finding that MTOC separation and amphiaster formation does not absolutely require the centrosome, but, in its absence, the fidelity of bipolar spindle assembly is highly compromised.
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