Evidence map›Paper›PMID 21439383›Full record

ArticleMethods (San Diego, Calif.)2011

Detection and characterization of ubiquitylated H2B in mammalian cells.

Efrat Shema, Moshe Oren, Neri Minsky

Open access · greenAbstract read
In one paragraph

Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Efrat ShemaDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. efrat.shema@weizmann.ac.il
Moshe Oren
Neri Minsky
Weizmann Institute of Science · ILRockefeller University · US

Funding

Functional analysis of the p53 tumor suppressorR37CA040099 · NCI · WEIZMANN INSTITUTE OF SCIENCE · PI OREN, MOSHE · 2003 to 2012
$2.2M
NCI NIH HHS R37 CA040099
6 · The paper itself

Abstract

Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.

Indexed as

Analytic Sample Preparation MethodsAnimalsAntibodies, MonoclonalBlotting, WesternCell ExtractsCell FusionCell NucleusCells, CulturedChromatinChromatin ImmunoprecipitationFemaleHistonesHybridomasMiceMice, Inbred BALB CPeptide FragmentsAntibodies, MonoclonalCell ExtractsChromatinHistonesPeptide FragmentsRecombinant Fusion ProteinsUbiquitinated Proteins

Identifiers

PMID21439383
PMCPMC3118924
OpenAlexW2043116216

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.