ArticleBMC plant biology2011
Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration.
Article in BMC plant biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 88 citations in OpenAlex.
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- Citrus Canker Pathogen, Its Mechanism of Infection, Eradication, and Impacts.Plants (Basel, Switzerland) · 2022Review
- The Citrus Laccase GeneInternational journal of molecular sciences · 2022Article
- Highly efficient hairy root genetic transformation and applications in citrus.Frontiers in plant science · 2022Article
- Genome-Wide Identification and Functional Analysis of Polyamine Oxidase Genes in Maize Reveal Essential Roles in Abiotic Stress Tolerance.Frontiers in plant science · 2022Article
- Novel resistance strategies to soybean cyst nematode (SCN) in wild soybean.Scientific reports · 2021Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- Characterization ofFrontiers in microbiology · 2021Article
- The unknown soldier in citrus plants: polyamines-based defensive mechanisms against biotic and abiotic stresses and their relationship with other stress-associated metabolites.Plant signaling & behavior · 2020Article
- Citrus Polyamines: Structure, Biosynthesis, and Physiological Functions.Plants (Basel, Switzerland) · 2020Review
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- SmPPT, a 4-hydroxybenzoate polyprenyl diphosphate transferase gene involved in ubiquinone biosynthesis, confers salt tolerance in Salvia miltiorrhiza.Plant cell reports · 2019Article
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
- The Interplay among Polyamines and Nitrogen in Plant Stress Responses.Plants (Basel, Switzerland) · 2019Review
- ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process.Plant biotechnology journal · 2019Article
- Polyamine Oxidases Play Various Roles in Plant Development and Abiotic Stress Tolerance.Plants (Basel, Switzerland) · 2019Review
- Contribution of Maize Polyamine and Amino Acid Metabolism Toward Resistance AgainstFrontiers in plant science · 2019Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene.
resultsIn the present work, an apple spermidine synthase gene (MdSPDS1) was introduced into sweet orange (Citrus sinensis Osbeck 'Anliucheng') via Agrobacterium-mediated transformation of embryogenic calluses. Two transgenic lines (TG4 and TG9) varied in the transgene expression and cellular endogenous polyamine contents. Pinprick inoculation demonstrated that the transgenic lines were less susceptible to Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus canker, than the wild type plants (WT). In addition, our data showed that upon Xac attack TG9 had significantly higher free spermine (Spm) and polyamine oxidase (PAO) activity when compared with the WT, concurrent with an apparent hypersensitive response and the accumulation of more H₂O₂. Pretreatment of TG9 leaves with guazatine acetate, an inhibitor of PAO, repressed PAO activity and reduced H₂O₂ accumulation, leading to more conspicuous disease symptoms than the controls when both were challenged with Xac. Moreover, mRNA levels of most of the defense-related genes involved in synthesis of pathogenesis-related protein and jasmonic acid were upregulated in TG9 than in the WT regardless of Xac infection.
conclusionOur results demonstrated that overexpression of the MdSPDS1 gene prominently lowered the sensitivity of the transgenic plants to canker. This may be, at least partially, correlated with the generation of more H₂O₂ due to increased production of polyamines and enhanced PAO-mediated catabolism, triggering hypersensitive response or activation of defense-related genes.
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