ArticleBMC plant biology2011
Glutathione synthesis is essential for pollen germination in vitro.
Article in BMC plant biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
- Exogenous Glutathione and Nitric Oxide Improve Waterlogging Stress Tolerance in Maize.Plant-environment interactions (Hoboken, N.J.) · 2026Article
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Glutathione S-transferaseFrontiers in plant science · 2025Article
- Analysis on morphological characteristics and identification of candidate genes during the flowering development of alfalfa.Frontiers in plant science · 2024Article
- Thermosensitivity of pollen: a molecular perspective.Plant cell reports · 2023Review
- Multi-omics research on common allergens during the ripening of pollen and poplar flocs ofFrontiers in plant science · 2023Article
- Integrated analysis of metabolome and transcriptome reveals the cytoplasmic effects of CMS-D2 on pollen fertility resulting from disrupted lipid metabolism.Frontiers in plant science · 2022Article
- A comprehensive integrated transcriptome and metabolome analyses to reveal key genes and essential metabolic pathways involved in CMS in kenaf.Plant cell reports · 2021Article
- Modulation of (Homo)Glutathione Metabolism and HInternational journal of molecular sciences · 2020Article
- Glutathione redox state plays a key role in flower development and pollen vigour.Journal of experimental botany · 2020Article
- Biotic priming withPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis.Journal of experimental botany · 2018Article
- A novel family of proline/serine-rich proteins, which are phospho-targets of stress-related mitogen-activated protein kinases, differentially regulates growth and pathogen defense in Arabidopsis thaliana.Plant molecular biology · 2017Article
- Untargeted metabolomic analysis of tomato pollen development and heat stress response.Plant reproduction · 2017Article
- Nitric Oxide and Reactive Oxygen Species Mediate Metabolic Changes in Barley Seed Embryo during Germination.Frontiers in plant science · 2016Article
- Unveiling the Redox Control of Plant Reproductive Development during Abiotic Stress.Frontiers in plant science · 2016Review
- Article
- Compartment specific response of antioxidants to drought stress in Arabidopsis.Plant science : an international journal of experimental plant biology · 2014Article
- Review
- Higher sensitivity of pad2-1 and vtc2-1 mutants to cadmium is related to lower subcellular glutathione rather than ascorbate contents.Protoplasma · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe antioxidant glutathione fulfills many important roles during plant development, growth and defense in the sporophyte, however the role of this important molecule in the gametophyte generation is largely unclear. Bioinformatic data indicate that critical control enzymes are negligibly transcribed in pollen and sperm cells. Therefore, we decided to investigate the role of glutathione synthesis for pollen germination in vitro in Arabidopsis thaliana accession Col-0 and in the glutathione deficient mutant pad2-1 and link it with glutathione status on the subcellular level.
resultsThe depletion of glutathione by buthionine sulfoximine (BSO), an inhibitor of glutathione synthesis, reduced pollen germination rates to 2-5% compared to 71% germination in wildtype controls. The application of reduced glutathione (GSH), together with BSO, restored pollen germination and glutathione contents to control values, demonstrating that inhibition of glutathione synthesis is responsible for the decrease of pollen germination in vitro. The addition of indole-3-acetic acid (IAA) to media containing BSO restored pollen germination to control values, which demonstrated that glutathione depletion in pollen grains triggered disturbances in auxin metabolism which led to inhibition of pollen germination.
conclusionsThis study demonstrates that glutathione synthesis is essential for pollen germination in vitro and that glutathione depletion and auxin metabolism are linked in pollen germination and early elongation of the pollen tube, as IAA addition rescues glutathione deficient pollen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.