ArticleBMC microbiology2011
The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- RpoS role in antibiotic resistance, tolerance and persistence in E. coli natural isolates.BMC microbiology · 2024Article
- Increased Levels of (p)ppGpp Correlate with Virulence and Biofilm Formation, but Not with Growth, in Strains of UropathogenicInternational journal of molecular sciences · 2023Article
- Fusion of the N-terminal 119 amino acids of RelA with the CTD domain render growth inhibitory effects of the latter, (p)ppGpp-dependent.Molecular genetics and genomics : MGG · 2022Article
- A Broad Continuum of E. coli Traits in Nature Associated with the Trade-off Between Self-preservation and Nutritional Competence.Microbial ecology · 2022Article
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- Dynamics of genetic variation in transcription factors and its implications for the evolution of regulatory networks in Bacteria.Nucleic acids research · 2020Article
- Different Dynamics of Bacterial and Fungal Communities in Hive-Stored Bee Bread and Their Possible Roles: A Case Study from Two Commercial Honey Bees in China.Microorganisms · 2020Article
- Diversity inFrontiers in microbiology · 2020Review
- Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels.Scientific reports · 2019Article
- Glyphosate induces the synthesis of ppGpp.Molecular genetics and genomics : MGG · 2019Article
- ppGpp and cytotoxicity diversity in Shiga toxin-producing Escherichia coli (STEC) isolates.Epidemiology and infection · 2017Article
- Genome-Wide Transcriptional Response to Varying RpoS Levels in Escherichia coli K-12.Journal of bacteriology · 2017Article
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- The General Stress Response Is Conserved in Long-Term Soil-Persistent Strains of Escherichia coli.Applied and environmental microbiology · 2016Article
- phoU inactivation in Pseudomonas aeruginosa enhances accumulation of ppGpp and polyphosphate.Applied and environmental microbiology · 2015Article
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- The rpoS gene is predominantly inactivated during laboratory storage and undergoes source-sink evolution in Escherichia coli species.Journal of bacteriology · 2014Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundSigma factors and the alarmone ppGpp control the allocation of RNA polymerase to promoters under stressful conditions. Both ppGpp and the sigma factor σS (RpoS) are potentially subject to variability across the species Escherichia coli. To find out the extent of strain variation we measured the level of RpoS and ppGpp using 31 E. coli strains from the ECOR collection and one reference K-12 strain.
resultsNine ECORs had highly deleterious mutations in rpoS, 12 had RpoS protein up to 7-fold above that of the reference strain MG1655 and the remainder had comparable or lower levels. Strain variation was also evident in ppGpp accumulation under carbon starvation and spoT mutations were present in several low-ppGpp strains. Three relationships between RpoS and ppGpp levels were found: isolates with zero RpoS but various ppGpp levels, strains where RpoS levels were proportional to ppGpp and a third unexpected class in which RpoS was present but not proportional to ppGpp concentration. High-RpoS and high-ppGpp strains accumulated rpoS mutations under nutrient limitation, providing a source of polymorphisms.
conclusionsThe ppGpp and σS variance means that the expression of genes involved in translation, stress and other traits affected by ppGpp and/or RpoS are likely to be strain-specific and suggest that influential components of regulatory networks are frequently reset by microevolution. Different strains of E. coli have different relationships between ppGpp and RpoS levels and only some exhibit a proportionality between increasing ppGpp and RpoS levels as demonstrated for E. coli K-12.
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