ArticleJournal of the American Chemical Society2011
Luminescent kinase activity biosensors based on a versatile bimolecular switch.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Sensitive and Selective Next-Generation FRET-based PKA Biosensors.bioRxiv : the preprint server for biology · 2026Article
- Sensitive fluorescent biosensor reveals differential subcellular regulation of PKC.Nature chemical biology · 2025Article
- Light-gated integrator for highlighting kinase activity in living cells.Nature communications · 2024Article
- Sensitive Fluorescent Biosensor Reveals Differential Subcellular Regulation of PKC.bioRxiv : the preprint server for biology · 2024Article
- Light-gated Integrator for Highlighting Kinase Activity in Living Cells.bioRxiv : the preprint server for biology · 2024Article
- Genetically Encoded Fluorescent Biosensors for Biomedical Applications.Biomedicines · 2021Review
- Genetically encoded sensors towards imaging cAMP and PKA activity in vivo.Journal of neuroscience methods · 2021Review
- An Integral Recognition and Signaling for Electrochemical Assay of Protein Kinase Activity and Inhibitor by Reduced Graphene Oxide-Polydopamine-Silver Nanoparticle-TiFrontiers in bioengineering and biotechnology · 2020Article
- Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer.The Journal of biological chemistry · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities.Nature cell biology · 2018Article
- Article
- Live-cell imaging of cell signaling using genetically encoded fluorescent reporters.The FEBS journal · 2018Review
- Signal Transduction at the Single-Cell Level: Approaches to Study the Dynamic Nature of Signaling Networks.Journal of molecular biology · 2016Review
- Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells.ACS chemical biology · 2016Article
- Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice.Theranostics · 2016Article
- Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARs.Chemistry & biology · 2015Article
- Genetically encoded molecular biosensors to image histone methylation in living animals.Analytical chemistry · 2015Article
- The role of dimerisation and nuclear transport in the Hes1 gene regulatory network.Bulletin of mathematical biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Real-time tracking of kinase activity in living systems has revealed new modes of encoding signaling information into spatiotemporal activity patterns and opened new avenues for screening kinase modulators. However, the sensitivity of kinase activity detection, which is commonly coupled to a fluorescence resonance energy transfer (FRET)-based readout, has often been a limiting factor. Here we show that a kinase-inducible bimolecular switch consisting of a substrate for the kinase of interest and a phosphoamino acid binding domain can be designed to sense different kinase activities and coupled to various readouts, thereby allowing for examination of dynamic kinase activity with increased sensitivity and versatility. Specifically, we demonstrate that bimolecular switches designed to sense protein kinase A (PKA) or protein kinase C (PKC) activities can turn on FRET as well as bioluminescence signals. Notably, the FRET-based sensors gain larger dynamic ranges in comparison with their unimolecular counterparts; the novel bioluminescence-based reporters for PKA and PKC show high sensitivity and a unique capability to detect basal kinase activities and should enable new applications in in vivo imaging of kinase activity and high-throughput compound screening. Thus, this generalizable design advances the molecular toolkit of kinase activity detection and provides a means for versatile and sensitive detection of kinase activity in various biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.