ArticlePloS one2011
What's new is old: resolving the identity of Leptothrix ochracea using single cell genomics, pyrosequencing and FISH.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 96 citations in OpenAlex.
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- Genomic Insights into Two Novel Fe(II)-OxidizingApplied and environmental microbiology · 2020Article
- The Bacterial Population of Neutral Mine Drainage Water of Elizabeth's Shaft (Slovinky, Slovakia).Current microbiology · 2018Article
- Insights into the Fundamental Physiology of the Uncultured Fe-Oxidizing Bacterium Leptothrix ochracea.Applied and environmental microbiology · 2018Article
- Diversity of Iron Oxidizers in Groundwater-Fed Rapid Sand Filters: Evidence of Fe(II)-Dependent Growth byFrontiers in microbiology · 2018Article
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- The Architecture of Iron Microbial Mats Reflects the Adaptation of Chemolithotrophic Iron Oxidation in Freshwater and Marine Environments.Frontiers in microbiology · 2016Article
- Coexistence of Microaerophilic, Nitrate-Reducing, and Phototrophic Fe(II) Oxidizers and Fe(III) Reducers in Coastal Marine Sediment.Applied and environmental microbiology · 2015Article
- Microbial iron oxidation in the Arctic tundra and its implications for biogeochemical cycling.Applied and environmental microbiology · 2015Article
- Novel and Unexpected Microbial Diversity in Acid Mine Drainage in Svalbard (78° N), Revealed by Culture-Independent Approaches.Microorganisms · 2015Article
- Preserved Filamentous Microbial Biosignatures in the Brick Flat Gossan, Iron Mountain, California.Astrobiology · 2015Article
- Microbial mineral colonization across a subsurface redox transition zone.Frontiers in microbiology · 2015Article
- Comparative Genomic Insights into Ecophysiology of Neutrophilic, Microaerophilic Iron Oxidizing Bacteria.Frontiers in microbiology · 2015Article
- The interplay of microbially mediated and abiotic reactions in the biogeochemical Fe cycle.Nature reviews. Microbiology · 2014Review
- Ecological succession among iron-oxidizing bacteria.The ISME journal · 2014Article
- Depth-dependent geochemical and microbiological gradients in Fe(III) deposits resulting from coal mine-derived acid mine drainage.Frontiers in microbiology · 2014Article
- Unraveling the stratification of an iron-oxidizing microbial mat by metatranscriptomics.PloS one · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Leptothrix ochracea is a common inhabitant of freshwater iron seeps and iron-rich wetlands. Its defining characteristic is copious production of extracellular sheaths encrusted with iron oxyhydroxides. Surprisingly, over 90% of these sheaths are empty, hence, what appears to be an abundant population of iron-oxidizing bacteria, consists of relatively few cells. Because L. ochracea has proven difficult to cultivate, its identification is based solely on habitat preference and morphology. We utilized cultivation-independent techniques to resolve this long-standing enigma. By selecting the actively growing edge of a Leptothrix-containing iron mat, a conventional SSU rRNA gene clone library was obtained that had 29 clones (42% of the total library) related to the Leptothrix/Sphaerotilus group (≤96% identical to cultured representatives). A pyrotagged library of the V4 hypervariable region constructed from the bulk mat showed that 7.2% of the total sequences also belonged to the Leptothrix/Sphaerotilus group. Sorting of individual L. ochracea sheaths, followed by whole genome amplification (WGA) and PCR identified a SSU rRNA sequence that clustered closely with the putative Leptothrix clones and pyrotags. Using these data, a fluorescence in-situ hybridization (FISH) probe, Lepto175, was designed that bound to ensheathed cells. Quantitative use of this probe demonstrated that up to 35% of microbial cells in an actively accreting iron mat were L. ochracea. The SSU rRNA gene of L. ochracea shares 96% homology with its closet cultivated relative, L. cholodnii, This establishes that L. ochracea is indeed related to this group of morphologically similar, filamentous, sheathed microorganisms.
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