ArticlePloS one2011
Characterization of bioactive recombinant human lysozyme expressed in milk of cloned transgenic cattle.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 144 citations in OpenAlex.
- Physicochemical Properties Determination of Recombinant Human Lysozyme and Its Effects on Intestinal Development in Mice.Nutrients · 2025Article
- The Role of Nutraceuticals in Age-Related Ocular Diseases.Molecules (Basel, Switzerland) · 2025Review
- Host-microbiota interactions in the infant gut revealed by daily faecal sample time series.Microbiome research reports · 2025Article
- Antimicrobial Properties of Colostrum and Milk.Antibiotics (Basel, Switzerland) · 2024Review
- Purification, Characterization and Bactericidal Action of Lysozyme, Isolated fromMolecules (Basel, Switzerland) · 2023Article
- Review
- Lysozyme and Its Application as Antibacterial Agent in Food Industry.Molecules (Basel, Switzerland) · 2022Review
- Effects of the thawing rate and heating temperature on immunoglobulin A and lysozyme activity in human milk.International breastfeeding journal · 2022Article
- Immunomodulatory Properties of Human Breast Milk: MicroRNA Contents and Potential Epigenetic Effects.Biomedicines · 2022Review
- An Overview of Antimicrobial Activity of Lysozyme and Its Functionality in Cheese.Frontiers in nutrition · 2022Review
- Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic.Antibiotics (Basel, Switzerland) · 2021Review
- Characterization of the regulatory 5'-flanking region of bovine mucin 2 (MUC2) gene.Molecular and cellular biochemistry · 2021Article
- Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle.International journal of molecular sciences · 2021Review
- Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals.International journal of molecular sciences · 2021Review
- Recent Insights Into the Prognostic and Therapeutic Applications of Lysozymes.Frontiers in pharmacology · 2021Review
- Article
- Lactic acid bacteria isolated from dairy products as potential producers of lipolytic, proteolytic and antibacterial proteins.Applied microbiology and biotechnology · 2019Article
- Applications of mesenchymal stem cell technology in bovine species.Stem cell research & therapy · 2019Review
- A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection.Journal of nanobiotechnology · 2019Article
- Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is great potential for using transgenic technology to improve the quality of cow milk and to produce biopharmaceuticals within the mammary gland. Lysozyme, a bactericidal protein that protects human infants from microbial infections, is highly expressed in human milk but is found in only trace amounts in cow milk. METHODOLOGY/PRINCIPAL
findingsWe have produced 17 healthy cloned cattle expressing recombinant human lysozyme using somatic cell nuclear transfer. In this study, we just focus on four transgenic cattle which were natural lactation. The expression level of the recombinant lysozyme was up to 25.96 mg/L, as measured by radioimmunoassay. Purified recombinant human lysozyme showed the same physicochemical properties, such as molecular mass and bacterial lysis, as its natural counterpart. Moreover, both recombinant and natural lysozyme had similar conditions for reactivity as well as for pH and temperature stability during in vitro simulations. The gross composition of transgenic and non-transgenic milk, including levels of lactose, total protein, total fat, and total solids were not found significant differences. CONCLUSIONS/SIGNIFICANCE: Thus, our study not only describes transgenic cattle whose milk offers the similar nutritional benefits as human milk but also reports techniques that could be further refined for production of active human lysozyme on a large scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.