ArticlePlant physiology2011
Protein phosphatase 2A B55 and A regulatory subunits interact with nitrate reductase and are essential for nitrate reductase activation.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Hyperosmolarity-induced suppression of group B1 Raf-like protein kinases modulates drought-growth trade-off inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- "B" Regulatory Subunits of PP2A: Their Roles in Plant Development and Stress Reactions.International journal of molecular sciences · 2023Review
- Exogenous Strigolactone (GR24) Positively Regulates Growth, Photosynthesis, and Improves Glandular Trichome Attributes for Enhanced Artemisinin Production inJournal of plant growth regulation · 2022Article
- Role of protein phosphatases in the regulation of nitrogen nutrition in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Posttranslational Modifications: Regulation of Nitrogen Utilization and Signaling.Plant & cell physiology · 2021Review
- Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass.Scientific reports · 2021Article
- Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants.Frontiers in plant science · 2020Article
- Effect of Different Rates of Nitrogen Fertilization on Crop Yield, Soil Properties and Leaf Physiological Attributes in Banana Under Subtropical Regions of China.Frontiers in plant science · 2020Article
- PP2A Phosphatases Take a Giant Leap in the Post-Genomics Era.Current genomics · 2019Review
- Article
- Article
- Copper amine oxidase 8 regulates arginine-dependent nitric oxide production in Arabidopsis thaliana.Journal of experimental botany · 2017Article
- Atypical Protein Phosphatase 2A Gene Families Do Not Expand via Paleopolyploidization.Plant physiology · 2017Article
- The effector AWR5 from the plant pathogen Ralstonia solanacearum is an inhibitor of the TOR signalling pathway.Scientific reports · 2016Article
- The Dual Role of Nitric Oxide in Guard Cells: Promoting and Attenuating the ABA and Phospholipid-Derived Signals Leading to the Stomatal Closure.Frontiers in plant science · 2016Article
- Protein phosphatase 2A holoenzyme is targeted to peroxisomes by piggybacking and positively affects peroxisomal β-oxidation.Plant physiology · 2015Article
- Arabidopsis PHOSPHOTYROSYL PHOSPHATASE ACTIVATOR is essential for PROTEIN PHOSPHATASE 2A holoenzyme assembly and plays important roles in hormone signaling, salt stress response, and plant development.Plant physiology · 2014Article
- Systems analysis of transcriptome data provides new hypotheses about Arabidopsis root response to nitrate treatments.Frontiers in plant science · 2014Article
- Evolution of bacterial-like phosphoprotein phosphatases in photosynthetic eukaryotes features ancestral mitochondrial or archaeal origin and possible lateral gene transfer.Plant physiology · 2013Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Posttranslational activation of nitrate reductase (NR) in Arabidopsis (Arabidopsis thaliana) and other higher plants is mediated by dephosphorylation at a specific Ser residue in the hinge between the molybdenum cofactor and heme-binding domains. The activation of NR in green leaves takes place after dark/light shifts, and is dependent on photosynthesis. Previous studies using various inhibitors pointed to protein phosphatases sensitive to okadaic acid, including protein phosphatase 2A (PP2A), as candidates for activation of NR. PP2As are heterotrimeric enzymes consisting of a catalytic (C), structural (A), and regulatory (B) subunit. In Arabidopsis there are five, three, and 18 of these subunits, respectively. By using inducible artificial microRNA to simultaneously knock down the three structural subunits we show that PP2A is necessary for NR activation. The structural subunits revealed overlapping functions in the activation process of NR. Bimolecular fluorescence complementation was used to identify PP2A regulatory subunits interacting with NR, and the two B55 subunits were positive. Interactions of NR and B55 were further confirmed by the yeast two-hybrid assay. In Arabidopsis the B55 group consists of the close homologs B55α and B55β. Interestingly, the homozygous double mutant (b55α × b55β) appeared to be lethal, which shows that the B55 group has essential functions that cannot be replaced by other regulatory subunits. Mutants homozygous for mutation in Bβ and heterozygous for mutation in Bα revealed a slower activation rate for NR than wild-type plants, pointing to these subunits as part of a PP2A complex responsible for NR dephosphorylation.
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