ArticleThe American journal of pathology2011
Genome and transcriptome profiles of CD133-positive colorectal cancer cells.
Article in The American journal of pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- CEACAM5 overexpression is a reliable characteristic of CD133-positive colorectal cancer stem cells.Cancer biomarkers : section A of Disease markers · 2021Article
- DNA alterations in Cd133+ and Cd133- tumour cells enriched from intra-operative human colon tumour biopsies.BMC cancer · 2017Article
- Genome-wide copy number changes and CD133 expression characterized distinct subset of colon polyps: differentiation between incidental polyps and cancer-associated polyps.International journal of colorectal disease · 2015Article
- Advanced colorectal adenoma related gene expression signature may predict prognostic for colorectal cancer patients with adenoma-carcinoma sequence.International journal of clinical and experimental medicine · 2015Article
- LGR5 positivity defines stem-like cells in colorectal cancer.Carcinogenesis · 2014Article
- Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells.PloS one · 2014Article
- Molecular patterns in the evolution of serrated lesion of the colorectum.International journal of cancer · 2013Article
- Chromothripsis and focal copy number alterations determine poor outcome in malignant melanoma.Cancer research · 2013Article
- Molecular pathways undergoing dramatic transcriptomic changes during tumor development in the human colon.BMC cancer · 2012Article
- [In situ hybridization in clinical pathology. Significance of polysomy 17 for HER2 determination and genetic tumor heterogeneity in breast cancer].Der Pathologe · 2012Review
- Rapid re-expression of CD133 protein in colorectal cancer cell lines in vitro and in vivo.Laboratory investigation; a journal of technical methods and pathology · 2012Article
- A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles.Translational oncology · 2012Article
- A new whole genome amplification method for studying clonal evolution patterns in malignant colorectal polyps.Genes, chromosomes & cancer · 2012Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
Abstract
Colorectal carcinomas (CRC) might be organized hierarchically and contain a subpopulation of tumorigenic, putative cancer stem cells that are CD133 positive. We studied the biological and genetic characteristics of such cells in CRC cell lines and primary tumors. Three CRC cell lines were sorted in CD133 positive and negative fractions. The respective genetic aberration profiles were studied using array comparative genomic hybridization (aCGH) and expression profiling. Tumorigenicity for each cellular population was tested by injection into nude mice. Additionally, we compared CD133+ and CD133- cells of 12 primary colorectal tumors using laser capture microdissection and aCGH. Three of five CRC cell lines displayed both CD133+ and CD133- cells, but tumorigenicity of these subfractions did not differ significantly and aCGH revealed essentially identical genomic imbalances. However, 96 genes were differentially expressed between the two populations. Array comparative genomic hybridization analysis after laser capture microdissection of CD133+ and CD133- areas in primary colorectal tumors revealed genetic differences in 7 of 12 cases. The use of cell lines for studying genomic alterations that define cancer stem cell characteristics, therefore, seems questionable. In contrast, CD133+ cells in primary cancer samples showed a unique genomic aberration profile. In conclusion, our data suggest that CD133 positivity defines a genetically distinct cellular compartment in primary CRC, which potentially includes tumor initiating cells.
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