ArticleMicrobial cell factories2011
Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli.
Article in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74 citing papers in PubMed, 189 citations in OpenAlex.
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
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- Concentration Recognition-Based Auto-Dynamic Regulation System (CRUISE) Enabling Efficient Production of Higher Alcohols.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Transcending membrane barriers: advances in membrane engineering to enhance the production capacity of microbial cell factories.Microbial cell factories · 2024Review
- Development of a co-culture system for green production of caffeic acid from sugarcane bagasse hydrolysate.Frontiers in microbiology · 2024Article
- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
- Escherichia coli displays a conserved membrane proteomic response to a range of alcohols.Biotechnology for biofuels and bioproducts · 2023Article
- Synthesis of isobutanol using acetate as sole carbon source in Escherichia coli.Microbial cell factories · 2023Article
- Optimizing the strain engineering process for industrial-scale production of bio-based molecules.Journal of industrial microbiology & biotechnology · 2023Review
- Recent progress on n-butanol production by lactic acid bacteria.World journal of microbiology & biotechnology · 2021Review
- Mutations in adaptively evolved Escherichia coli LGE2 facilitated the cost-effective upgrading of undetoxified bio-oil to bioethanol fuel.Bioresources and bioprocessing · 2021Article
- EvolvingInternational journal of molecular sciences · 2021Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Characterizing Escherichia coli's transcriptional response to different styrene exposure modes reveals novel toxicity and tolerance insights.Journal of industrial microbiology & biotechnology · 2021Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Article
- Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw material.Journal of industrial microbiology & biotechnology · 2020Article
- Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol.Biotechnology for biofuels · 2020Article
- Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides.Biotechnology and bioengineering · 2020Article
- Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIsobutanol is a promising next-generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titer. Organic solvent tolerance is a complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing and a gene expression study to elucidate genetic bases of adaptation to exogenous isobutanol stress.
resultsThe adaptations acquired in our evolved lineages exhibit antagonistic pleiotropy between minimal and rich medium, and appear to be specific to the effects of longer chain alcohols. By examining genotypic adaptation in multiple independent lineages, we find evidence of parallel evolution in marC, hfq, mdh, acrAB, gatYZABCD, and rph genes. Many isobutanol tolerant lineages show reduced RpoS activity, perhaps related to mutations in hfq or acrAB. Consistent with the complex, multigenic nature of solvent tolerance, we observe adaptations in a diversity of cellular processes. Many adaptations appear to involve epistasis between different mutations, implying a rugged fitness landscape for isobutanol tolerance. We observe a trend of evolution targeting post-transcriptional regulation and high centrality nodes of biochemical networks. Collectively, the genotypic adaptations we observe suggest mechanisms of adaptation to isobutanol stress based on remodeling the cell envelope and surprisingly, stress response attenuation.
conclusionsWe have discovered a set of genotypic adaptations that confer increased tolerance to exogenous isobutanol stress. Our results are immediately useful to further efforts to engineer more isobutanol tolerant host strains of E. coli for isobutanol production. We suggest that rpoS and post-transcriptional regulators, such as hfq, RNA helicases, and sRNAs may be interesting mutagenesis targets for future global phenotype engineering.
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