ArticleBMC genomics2011
Proteomic analysis of the marine diatom Thalassiosira pseudonana upon exposure to benzo(a)pyrene.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Microbial response and resistance mechanisms against diverse anthropogenic pollutants.Frontiers in microbiology · 2026Review
- The Ratio of Silicon to Chitin in Diatom Cell Walls Influences Their Resilience to Environmental Stress.Research (Washington, D.C.) · 2026Article
- Article
- Molecular mechanism of oil induced growth inhibition in diatoms using Thalassiosira pseudonana as the model species.Scientific reports · 2021Article
- Influence of nutrient status on the response of the diatom Phaeodactylum tricornutum to oil and dispersant.PloS one · 2021Article
- Inhibition of orf virus replication in goat skin fibroblast cells by the HSPA1B protein, as demonstrated by iTRAQ-based quantitative proteome analysis.Archives of virology · 2020Article
- Quantitative Proteomic Profiling of Marine DiatomFrontiers in microbiology · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Environmental fluctuations accelerate molecular evolution of thermal tolerance in a marine diatom.Nature communications · 2018Article
- Identification and Characterization of Differentially-Regulated Type IVb Pilin Genes Necessary for Predation in Obligate Bacterial Predators.Scientific reports · 2017Article
- High light stress triggers distinct proteomic responses in the marine diatom Thalassiosira pseudonana.BMC genomics · 2016Article
- Proteome response ofAlgal research · 2016Article
- Proteomic analysis of plasma membrane proteins in wheat roots exposed to phenanthrene.Environmental science and pollution research international · 2016Article
- iTRAQ-based quantitative proteomic analysis reveals the distinct early embryo myofiber type characteristics involved in landrace and miniature pig.BMC genomics · 2016Article
- Differential cellular metabolite alterations in HaCaT cells caused by exposure to the aryl hydrocarbon receptor-binding polycyclic aromatic hydrocarbons chrysene, benzo[Toxicology reports · 2016Article
- iTRAQ-based proteomic profiling of Vibrio parahaemolyticus under various culture conditions.Proteome science · 2015Article
- Metabolomics and proteomics reveal impacts of chemically mediated competition on marine plankton.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Understanding the sub-cellular dynamics of silicon transportation and synthesis in diatoms using population-level data and computational optimization.PLoS computational biology · 2014Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolycyclic aromatic hydrocarbons (PAHs) are environmental pollutants ubiquitously distributed. They are generated by incomplete combustion of organic materials such as wood or fossil fuels. Due to their carcinogenic, mutagenic effects and to their wide distribution in the environment, these pollutants pose many concerns to researchers and regulators. In our laboratories we investigated the effect of benzo(a)pyrene (BaP) exposure in the marine diatom Thalassiosira pseudonana, which has become an important model organism in aquatic toxicology studies.
resultsIn order to investigate the mechanism of action of PAHs, we exposed the diatoms for 24 h to 36.45 μg/L of BaP which inhibits the growth by about 30%, and analysed the relative protein expression profile by a quantitative proteomics approach based on iTRAQ labels. The proteomics profile analysis showed that around 10% of the identified proteins were regulated and one fourth of them confirmed the gene expression changes seen by DNA microarray. Particularly interesting was the down regulation of the Silicon transporter 1 (SIT1), an enzyme that is responsible for the uptake of silicon from the media into the diatom cells. Regulation of SIT1 upon BaP treatment was also confirmed at the gene expression level.
conclusionsThe potential use of the regulated proteins found in this study as early indicators of environmental exposure to PAHs is discussed. In particular, SIT1 is considered a promising biomarker and SIT1 expression changes were confirmed also when the diatoms were exposed to field samples, e.g. marine surface sediments contaminated by PAHs.
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