ArticleThe Journal of cell biology1990
Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Article
- Article
- Evidence for monomeric actin function in INO80 chromatin remodeling.Nature structural & molecular biology · 2013Article
- Actin filaments as tension sensors.Current biology : CB · 2012Review
- Remodeling of actin filaments by ADF/cofilin proteins.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Myosin binding surface on actin probed by hydroxyl radical footprinting and site-directed labels.Journal of molecular biology · 2011Article
- Structural polymorphism in F-actin.Nature structural & molecular biology · 2010Article
- Mutation of actin Tyr-53 alters the conformations of the DNase I-binding loop and the nucleotide-binding cleft.The Journal of biological chemistry · 2010Article
- F-actin structure destabilization and DNase I binding loop: fluctuations mutational cross-linking and electron microscopy analysis of loop states and effects on F-actin.Journal of molecular biology · 2010Article
- D-loop of actin differently regulates the motor function of myosins II and V.The Journal of biological chemistry · 2009Article
- Large-scale models reveal the two-component mechanics of striated muscle.International journal of molecular sciences · 2008Article
- Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Actin depolymerizing factor stabilizes an existing state of F-actin and can change the tilt of F-actin subunits.The Journal of cell biology · 2001Article
- Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor.Biophysical journal · 2001Article
- F-actin retains a memory of angular order.Biophysical journal · 2000Article
- Intermolecular coupling between loop 38-52 and the C-terminus in actin filaments.Biophysical journal · 1996Article
- Myosin-induced changes in F-actin: fluorescence probing of subdomain 2 by dansyl ethylenediamine attached to Gln-41.Biophysical journal · 1996Article
- end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- Orientation of actin filaments during motion in in vitro motility assay.Biophysical journal · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Subtilisin cleaved actin was shown to retain several properties of intact actin including the binding of heavy meromyosin (HMM), the dissociation from HMM by ATP, and the activation of HMM ATPase activity. Similar Vmax but different Km values were obtained for acto-HMM ATPase with the cleaved and intact actins. The ATPase activity of HMM stimulated by copolymers of intact and cleaved actin showed a linear dependence on the fraction of intact actin in the copolymer. The most important difference between the intact and cleaved actin was observed in an in vitro motility assay for actin sliding movement over an HMM coated surface. Only 30% of the cleaved actin filaments appeared mobile in this assay and moreover, the velocity of the mobile filaments was approximately 30% that of intact actin filaments. These results suggest that the motility of actin filaments can be uncoupled from the activation of myosin ATPase activity and is dependent on the structural integrity of actin and perhaps, dynamic changes in the actin molecule.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.