Evidence map›Paper›PMID 21431911›Full record

ArticleFolia microbiologica2011

Molecular analysis and characterization of a urease gene operon from Campylobacter sputorum biovar paraureolyticus.

Y Kakinuma, K Hayashi, A Tazumi, J Hirayama, J E Moore, B C Millar, T Kuribayashi, M Matsuda

Abstract read
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Article in Folia microbiologica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Y KakinumaGraduate School of Environmental Health Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku Sagamihara, 252-5201, Japan.
K Hayashi
A Tazumi
J Hirayama
J E Moore
B C Millar
T Kuribayashi
M Matsuda

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

When recombinant plasmid DNA from a genomic DNA library and inverse PCR products of Campylobacter sputorum biovar paraureolyticus LMG17591 strain were analyzed, an approximate 6.5-kb pair region, encoding a urease gene operon, was identified. Within the operon, seven closely spaced and putative open reading frames for ureG, ureH(D), ureA, ureB, ureC, ureE, and ureF were detected in order. A possible overlap was detected between ureG and ureH(D), ureH(D) and ureA, and ureE and ureF. In addition, two putative promoter structures, probable ribosome-binding sites and a putative ρ-independent transcriptional terminator structure were identified. The urease gene operon transcription in the cells was confirmed by the reverse transcription-PCR analysis. A neighbor-joining tree constructed based on the nucleotide sequence information of urease genes showed that C. sputorum biovar paraureolyticus formed a cluster with Arcobacter butzleri, urease-positive thermophilic Campylobacter and some Helicobacter spp., separating those from the other urease-producing bacteria, suggesting a commonly shared ancestry among these organisms.

Indexed as

OperonAnimalsCampylobacter sputorumCattleCluster AnalysisDNA, BacterialGene Expression ProfilingGene LibraryGene OrderMolecular Sequence DataOpen Reading FramesPhylogenyPromoter Regions, GeneticSequence Analysis, DNASequence Homology, Nucleic AcidTerminator Regions, GeneticDNA, BacterialUrease

Identifiers

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.