ArticleMethods in molecular biology (Clifton, N.J.)2011
Transcriptome profiling using single-molecule direct RNA sequencing.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Nanopore direct RNA sequencing and the epitranscriptome: Advances in mapping native RNA landscapes.iMeta · 2026Review
- The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology.Biosensors · 2024Review
- Transcriptome profiling for developmental stages Protaetia brevitarsis seulensis with focus on wing development and metamorphosis.PloS one · 2023Article
- Third-Generation Sequencing: The Spearhead towards the Radical Transformation of Modern Genomics.Life (Basel, Switzerland) · 2021Review
- The Detection and Bioinformatic Analysis of Alternative 3International journal of molecular sciences · 2021Review
- Regulatory roles ofeLife · 2021Article
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Evaluation of changes to the Rickettsia rickettsii transcriptome during mammalian infection.PloS one · 2017Article
- A Genome-wide Study of "Non-3UTR" Polyadenylation Sites in Arabidopsis thaliana.Scientific reports · 2016Article
- Biochemical Methods To Investigate lncRNA and the Influence of lncRNA:Protein Complexes on Chromatin.Biochemistry · 2016Review
- Transcriptomic Sequencing Reveals a Set of Unique Genes Activated by Butyrate-Induced Histone Modification.Gene regulation and systems biology · 2016Article
- Whole transcriptome analysis with sequencing: methods, challenges and potential solutions.Cellular and molecular life sciences : CMLS · 2015Review
- Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq.BMB reports · 2015Article
- Hypothesis: Artifacts, Including Spurious Chimeric RNAs with a Short Homologous Sequence, Caused by Consecutive Reverse Transcriptions and Endogenous Random Primers.Journal of Cancer · 2015Review
- RNA-Seq technology and its application in fish transcriptomics.Omics : a journal of integrative biology · 2014Review
- sRNAs and the virulence of Salmonella enterica serovar Typhimurium.RNA biology · 2012Review
- Sequencing technologies and genome sequencing.Journal of applied genetics · 2011Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Methods for in-depth characterization of transcriptomes and quantification of transcript levels have emerged as valuable tools for understanding cellular physiology and human disease biology, and have begun to be utilized in various clinical diagnostic applications. Today, current methods utilized by the scientific community typically require RNA to be converted to cDNA prior to comprehensive measurements. However, this cDNA conversion process has been shown to introduce many biases and artifacts that interfere with the proper characterization and quantitation of transcripts. We have developed a direct RNA sequencing (DRS) approach, in which, unlike other technologies, RNA is sequenced directly without prior conversion to cDNA. The benefits of DRS include the ability to use minute quantities (e.g. on the order of several femtomoles) of RNA with minimal sample preparation, the ability to analyze short RNAs which pose unique challenges for analysis using cDNA-based approaches, and the ability to perform these analyses in a low-cost and high-throughput manner. Here, we describe the strategies and procedures we employ to prepare various RNA species for analysis with DRS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.